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- W3143157207 abstract "A fluorometric assay was used to study the DNA unwinding kinetics induced by Escherichia coli RecQ helicase.This assay was based on fluorescence resonance energy transfer and carried out on stopped-flow,in which DNA unwinding was monitored by fluorescence emission enhancement of fluorescein resulting from helicase-catalyzed DNA unwinding.By this method,we determined the DNA unwinding rate of RecQ at different enzyme concentrations.We also studied the dependences of DNA unwinding magnitude and rate on magnesium ion concentration.We showed that this method could be used to determine the polarity of DNA unwinding.This assay should greatly facilitate further study of the mechanism for RecQ- catalyzed DNA unwinding." @default.
- W3143157207 created "2021-04-13" @default.
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- W3143157207 date "2005-01-01" @default.
- W3143157207 modified "2023-09-23" @default.
- W3143157207 title "RecQ Helicase-catalyzed DNA Unwinding Detected by Fluorescence Resonance Energy Transfer" @default.
- W3143157207 hasPublicationYear "2005" @default.
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