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• W3143296072 abstract "For efficient transgenic herd expansion, only the transgenic animals that possess the ability to transmit transgene into next generation are considered for breeding. However, for transgenic pig, practically lacking a pre-breeding screening program, time, labor and money is always wasted to maintain non-trans- genic pigs, low or null transgenic transmission pigs and the related fruitless gestations. Developing a pre- breeding screening program would make the trans- genic herd expansion more economical and efficient. In this technical report, we proposed a three-step pre- breeding screening program for transgenic boars simply through combining the fluorescence in situ hybridization (FISH) assay with the common pre- breeding screening workflow. In the first step of screening, combined with general transgenic pheno- type analysis, FISH is used to identify transgenic boars. In the second step of screening, combined with conventional semen test, FISH is used to detect transgenic sperm, thus to identify the individuals producing high quality semen and transgenic sperm. In the third step of screening, FISH is used to assess the in vitro fertilization embryos, thus finally to identify the individuals with the ability to produce transgenic embryos. By this three-step screening, the non-trans- genic boars and boars with no ability to produce transgenic sperm or transgenic embryos would be eliminated; therefore only those boars could produce transgenic offspring are maintained and used for breeding and herd expansion. It is the first time a systematic pre-breeding screening program is pro- posed for transgenic pigs. This program might also be applied in other transgenic large animals, and provide an economical and efficient strategy for herd expansion." @default.
• W3143296072 created "2021-04-13" @default.
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• W3143296072 date "2014-01-01" @default.
• W3143296072 modified "2023-09-24" @default.
• W3143296072 title "A pre-breeding screening program for transgenic boars based on fluorescence in situ hybridization assay" @default.
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• W3143296072 hasPublicationYear "2014" @default.
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