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- W3143404837 abstract "Background: OA affects over 200 million people across the globe and the number is only expected to increase over the next few decades. It is a health burden that involves the degradation and degeneration of the cartilage in the joints between the bones. The end point treatment is normally surgery after using a prosthetic implant to restore joint function. Thus, there is a need to focus on treatment approaches that can halt the progression of the disease at an earlier stage. Research involving cell therapies, especially using mesenchymal stem cells have gained a lot of interest owing to their ability to differentiate into various kinds of cells. However, they need to be supported with a system that allows them to survive and maintain their function. This was done with the help of biomaterials that have the ability to interact with biological systems around them. Methods: We aimed to use a method called the layer-by-layer (LBL) approach to modify biomaterial surfaces that would influence cell activity. We also aimed at fabricating micro-scale structures for using in the development of self-assembled matrices that would support cellular activity. LBL using the dip-coating method was used for both; modifying the biomaterial to create an extracellular matrix (ECM) like system with Type I Collagen and Hyaluronic Acid (Type I Coll/HA) for cells to organize themselves, as well as for developing self-assembled micro-tubes with PAH [FITC (poly allyl amine hydrochloride)] and PSS [poly (sodium-4-styrenesulphonate)] for providing support to the cells. The tubes were then fabricated with Streptavidin and were coated with complementary biotin oligonucleotides. The concept of strong bond between streptavidin-biotin bond was used to see if the tubes fabricated could be used for building scaffold like structures for cells to organize themselves. Results: On viewing the cells under the scanning electron microscope (SEM) we found that human mesenchymal stem cells had organized themselves on the complex ECM like matrix that was built for them and the biomaterial fabricated was able to influence cell behaviour. We also found that the micro structures fabricated had the potential to give rise to a cross-linked scaffold system that would support cell structure and activity. Conclusion: After having a look at the results and discussion it can be concluded that the human mesenchymal stem cells were found to have better adhesion to the complex matrix that was prepared by LBL. The fabricated micro tubes could possibly be used in the future to give rise to biomaterial scaffolds to influence cell behaviour and characteristics. The micro tubes did seem to have the potential to interact with the biological system surrounding them. Disclosure statement: The authors have declared no conflicts of interest." @default.
- W3143404837 created "2021-04-13" @default.
- W3143404837 date "2005-03-01" @default.
- W3143404837 modified "2023-09-26" @default.
- W3143404837 title "Osteoporosis and metabolic bone disease [3-19]" @default.
- W3143404837 doi "https://doi.org/10.1093/rheumatology/44.suppl_1.i30-a" @default.
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