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- W3143450598 abstract "One adult α-globin gene and one β-globin gene have been cloned from the large yellow croaker Pseudosciaena crocea. Linkage analysis indicated that the α-and β-globin genes were oriented head-to-head relative to each other. To identify the regulatory elements present in the intergenic and intragenic regions of the globin complex,the intergenic region alone or together with the β-globin gene first intron was cloned into the luciferase-reporter vector pGL3-Basic respectively,and the chimeric constructs were transiently transfected into Vero cells and primary fish erythrocytes. The intergenic region cannot support the high-level expression of luciferase. However,the promoter activity of the intergenic region was strongly stimulated by the positive regulatory elements (PRE) located in the β-globin gene intron 1. Thus,it is proposed that the intergenic promoters and intragenic PRE were necessary for the effective expression of the linked α-and β-globin genes." @default.
- W3143450598 created "2021-04-13" @default.
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- W3143450598 date "2008-01-01" @default.
- W3143450598 modified "2023-09-23" @default.
- W3143450598 title "Intragenic and intergenic sequences regulating the expression of the 5'-to-5' linked adult α-and β-globin genes from large yellow croaker Pseudosciaena crocea" @default.
- W3143450598 hasPublicationYear "2008" @default.
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