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- W3143467221 abstract "Cultured porcine aortic endothelial cells (EC) secrete a potent mitogen into their serum-free culture medium which stimulates DNA synthesis in quiescent human fibroblasts. Previous work has shown that endothelial cell-conditioned medium (ECCM) also stimulates parallel, concentration-dependent increases in low density lipoprotein (LDL) cell surface binding and internalization. LDL degradation, however, was inhibited in ECCM-treated cells. Studies were conducted to determine whether the EC-derived growth factor (EDGF) and the endothelial-derived inhibitor of LDL degradation (EDID) are separate and distinct components, and to further delineate some of the characteristics of EDID. Time-course studies showed that the mitogenic activity released by EC into ECCM increased steadily during the first 24 hours and came to a plateau at about 48 hours of conditioning, whereas inhibitory activity increased linearly in ECCM for at least 6 days. Mitogenic activity in ECCM was stable under the same conditions. Ultrafiltra..." @default.
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- W3143467221 date "1983-01-01" @default.
- W3143467221 modified "2023-09-24" @default.
- W3143467221 title "Inhibition of LDL degradation in cultured human fibroblasts induced by endothelial cell-conditioned medium." @default.
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