FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3143559155> ?p ?o ?g. }

• W3143559155 abstract "Objective To investigate the protection and mechanism of lactulose preconditioning on liver ischemia-reperfusion injury(IRI) in rats. Methods Forty-five Sprague-Dawley rats were randomly divided into three groups by random number table : sham-operation( SO) group, IRI group, lactulose preconditioning(lactulose) group with 15 rats in each group. Lactulose(0.1 g/ml)was administered daily via gavage in lactulose group 7 d before operation, while equal volume of normal saline was administered via gavage in IRI and SO group. In IRI and lactulose group, the portal vein and corresponding hepatic artery branches of middle and left hepatic lobes were clamped with bulldogs clamps during the operation to make 70% of the liver in ischemia, and blood supply recovered after 60 min′ s blocking, and reperfusion wasperformed for 6 h to establish IRI model. In SO group, the rats only underwent open surgery and hepatic ligaments were exposed and separated. After operation, the levels of serum interleukin(IL)-1β, IL-6 and tumor necrosis factor(TNF)-α were detected by enzyme linked immunosorbent assay. The morphological change of liver tissue was observed under light microscope. Hepatocyte apoptosis was observed under fluorescence microscope. The expression of activated caspase-3 was detected by Western blot. The detecting data of cytokines in rats among 3 groups were compared by one-way analysis of variance and LSD-t test. Results The level of IL-1β in SO, IRI and lactulose group was(75 ±22),(365 ±30),(139 ±16)ng/L respectively, and the level of IL-6 was(48±16),(190±22),(92±10)ng/L respectively, and the level of TNF-α was(11.3±5.1),(40.2±2.7),(19.5±3.9)ng/L respectively. The levels of IL-1β, IL-6, TNF-α in IRI group were significantly higher than those of SO group(LSD-t=30.6,20.6,19.4; P0.05), while were significantly lower than those of lactulose group(LSD-t=-26.2,-16.1,-16.9; P0.05). Normal morphology of liver tissue was observed in SO group. Large area of focal or patchy necrosis of hepatocytes was observed in IRI group. Hydropic degeneration and spotty necrosis were observed in lactulose group, and its tissue structure damage was milder than that of IRI group. No obvious apoptosis was observed in SO group. The number of apoptosis obviously increased in lactulose and IRI group, while the number of apoptosis in lactulose group was less than that of IRI group. Compared with the SO group, the expression of activated caspase-3 in lactulose group was up-regulated, but more down-regulated than the IRI group. Conclusion Lactulose preconditioning can alleviate liver IRI in rats, which may be related with the inhibition of inflammatory cytokine release and the inhibition of apoptosis." @default.
• W3143559155 created "2021-04-13" @default.
• W3143559155 creator A5010222868 @default.
• W3143559155 date "2013-01-01" @default.
• W3143559155 modified "2023-09-27" @default.
• W3143559155 title "Protection of lactulose preconditioning on liver ischemia-reperfusion injury in rats" @default.
• W3143559155 hasPublicationYear "2013" @default.
• W3143559155 type Work @default.
• W3143559155 sameAs 3143559155 @default.
• W3143559155 citedByCount "0" @default.
• W3143559155 crossrefType "journal-article" @default.
• W3143559155 hasAuthorship W3143559155A5010222868 @default.
• W3143559155 hasConcept C126322002 @default.
• W3143559155 hasConcept C142724271 @default.
• W3143559155 hasConcept C17991360 @default.
• W3143559155 hasConcept C2777397205 @default.
• W3143559155 hasConcept C2777448540 @default.
• W3143559155 hasConcept C2779311647 @default.
• W3143559155 hasConcept C2780114680 @default.
• W3143559155 hasConcept C541997718 @default.
• W3143559155 hasConcept C71924100 @default.
• W3143559155 hasConcept C90924648 @default.
• W3143559155 hasConceptScore W3143559155C126322002 @default.
• W3143559155 hasConceptScore W3143559155C142724271 @default.
• W3143559155 hasConceptScore W3143559155C17991360 @default.
• W3143559155 hasConceptScore W3143559155C2777397205 @default.
• W3143559155 hasConceptScore W3143559155C2777448540 @default.
• W3143559155 hasConceptScore W3143559155C2779311647 @default.
• W3143559155 hasConceptScore W3143559155C2780114680 @default.
• W3143559155 hasConceptScore W3143559155C541997718 @default.
• W3143559155 hasConceptScore W3143559155C71924100 @default.
• W3143559155 hasConceptScore W3143559155C90924648 @default.
• W3143559155 hasLocation W31435591551 @default.
• W3143559155 hasOpenAccess W3143559155 @default.
• W3143559155 hasPrimaryLocation W31435591551 @default.
• W3143559155 hasRelatedWork W2350198497 @default.
• W3143559155 hasRelatedWork W2358405068 @default.
• W3143559155 hasRelatedWork W2360822729 @default.
• W3143559155 hasRelatedWork W2375125288 @default.
• W3143559155 hasRelatedWork W2381009603 @default.
• W3143559155 hasRelatedWork W2385779299 @default.
• W3143559155 hasRelatedWork W2386227562 @default.
• W3143559155 hasRelatedWork W2901813818 @default.
• W3143559155 hasRelatedWork W3029596656 @default.
• W3143559155 hasRelatedWork W3030542384 @default.
• W3143559155 hasRelatedWork W3030885158 @default.
• W3143559155 hasRelatedWork W3031039740 @default.
• W3143559155 hasRelatedWork W3031461931 @default.
• W3143559155 hasRelatedWork W3031548395 @default.
• W3143559155 hasRelatedWork W3031602670 @default.
• W3143559155 hasRelatedWork W3031648314 @default.
• W3143559155 hasRelatedWork W3031714264 @default.
• W3143559155 hasRelatedWork W3032825489 @default.
• W3143559155 hasRelatedWork W3148445403 @default.
• W3143559155 hasRelatedWork W3032347238 @default.
• W3143559155 isParatext "false" @default.
• W3143559155 isRetracted "false" @default.
• W3143559155 magId "3143559155" @default.
• W3143559155 workType "article" @default.