Matches in SemOpenAlex for { <https://semopenalex.org/work/W3143609276> ?p ?o ?g. }
Showing items 1 to 73 of
73
with 100 items per page.
- W3143609276 abstract "This thesis describes efforts in the development of an immobilised enzyme biocatalyst for a scaleable biotransformation. The enzyme transketolase, which catalyses the synthesis of carbon-carbon bonds, was chosen as a model. The enzyme has been cloned into E.coli and can be produced on a large scale and is consequently representative of an industrial enzyme. Transketolase has been immobilised onto the commercially available materials Eupergit-C and Amberlite XAD7. With a binding yield of ca 90 % and a retention of activity between 20 % and 60 % depending upon enzyme load in the case of Eupergit-C and a binding yield of 90 % and a retention of 20% activity in the case of Amberlite XAD7. The cofactor thiamine pyrophosphate was successfully introduced to the Eupergit-C immobilised enzyme, by co-immobilising the enzyme and cofactor, to yield a biocatalyst with a specific activity of 840 U.g-1 and a retention of activity of 58 %. Although the cofactor was found to leach from the immobilised holo-enzyme as a consequence excess cofactor had to be maintained within the bioreactor environment. Both immobilised enzymes were characterised and an improvement in the stability of both the Eupergit-C and Amberlite XAD7 preparations against the toxic substrate glycolaldehyde was observed. The half life in the presence of 0.5 M glycolaldehyde was improved from 1 hour to 100 hours and 80 hours in the case of Eupergit-C and Amberlite XAD7 respectively. An economic comparison of Eupergit-C and Amberlite XAD7 was made and Eupergit-C was found to be the more economic support material producing a biocatalyst with a space time yield of 674 kg.L-1 /batch and a calculated enzyme productivity of 100kU/kg product after 20 batches." @default.
- W3143609276 created "2021-04-13" @default.
- W3143609276 creator A5057103482 @default.
- W3143609276 date "2000-01-01" @default.
- W3143609276 modified "2023-09-23" @default.
- W3143609276 title "Preparation and characterisation of biocompatible magnetic fluids." @default.
- W3143609276 hasPublicationYear "2000" @default.
- W3143609276 type Work @default.
- W3143609276 sameAs 3143609276 @default.
- W3143609276 citedByCount "0" @default.
- W3143609276 crossrefType "dissertation" @default.
- W3143609276 hasAuthorship W3143609276A5057103482 @default.
- W3143609276 hasConcept C103432212 @default.
- W3143609276 hasConcept C134121241 @default.
- W3143609276 hasConcept C150394285 @default.
- W3143609276 hasConcept C154881586 @default.
- W3143609276 hasConcept C161790260 @default.
- W3143609276 hasConcept C178790620 @default.
- W3143609276 hasConcept C181199279 @default.
- W3143609276 hasConcept C185592680 @default.
- W3143609276 hasConcept C191503008 @default.
- W3143609276 hasConcept C191897082 @default.
- W3143609276 hasConcept C192562407 @default.
- W3143609276 hasConcept C197957613 @default.
- W3143609276 hasConcept C2776651935 @default.
- W3143609276 hasConcept C2778699723 @default.
- W3143609276 hasConcept C2780047943 @default.
- W3143609276 hasConcept C33633552 @default.
- W3143609276 hasConcept C43617362 @default.
- W3143609276 hasConceptScore W3143609276C103432212 @default.
- W3143609276 hasConceptScore W3143609276C134121241 @default.
- W3143609276 hasConceptScore W3143609276C150394285 @default.
- W3143609276 hasConceptScore W3143609276C154881586 @default.
- W3143609276 hasConceptScore W3143609276C161790260 @default.
- W3143609276 hasConceptScore W3143609276C178790620 @default.
- W3143609276 hasConceptScore W3143609276C181199279 @default.
- W3143609276 hasConceptScore W3143609276C185592680 @default.
- W3143609276 hasConceptScore W3143609276C191503008 @default.
- W3143609276 hasConceptScore W3143609276C191897082 @default.
- W3143609276 hasConceptScore W3143609276C192562407 @default.
- W3143609276 hasConceptScore W3143609276C197957613 @default.
- W3143609276 hasConceptScore W3143609276C2776651935 @default.
- W3143609276 hasConceptScore W3143609276C2778699723 @default.
- W3143609276 hasConceptScore W3143609276C2780047943 @default.
- W3143609276 hasConceptScore W3143609276C33633552 @default.
- W3143609276 hasConceptScore W3143609276C43617362 @default.
- W3143609276 hasLocation W31436092761 @default.
- W3143609276 hasOpenAccess W3143609276 @default.
- W3143609276 hasPrimaryLocation W31436092761 @default.
- W3143609276 hasRelatedWork W1990256165 @default.
- W3143609276 hasRelatedWork W1992634272 @default.
- W3143609276 hasRelatedWork W2002695448 @default.
- W3143609276 hasRelatedWork W2016521270 @default.
- W3143609276 hasRelatedWork W2036537664 @default.
- W3143609276 hasRelatedWork W2040686300 @default.
- W3143609276 hasRelatedWork W2048417074 @default.
- W3143609276 hasRelatedWork W2070818249 @default.
- W3143609276 hasRelatedWork W2087513387 @default.
- W3143609276 hasRelatedWork W2092277385 @default.
- W3143609276 hasRelatedWork W2109709377 @default.
- W3143609276 hasRelatedWork W2113173689 @default.
- W3143609276 hasRelatedWork W2120736568 @default.
- W3143609276 hasRelatedWork W2128576027 @default.
- W3143609276 hasRelatedWork W2378644226 @default.
- W3143609276 hasRelatedWork W2383456536 @default.
- W3143609276 hasRelatedWork W2464862821 @default.
- W3143609276 hasRelatedWork W2618346983 @default.
- W3143609276 hasRelatedWork W3009894462 @default.
- W3143609276 hasRelatedWork W2846066066 @default.
- W3143609276 isParatext "false" @default.
- W3143609276 isRetracted "false" @default.
- W3143609276 magId "3143609276" @default.
- W3143609276 workType "dissertation" @default.