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• W3143643313 abstract "1746-Pos Board B638 Correlative 3-Dimensional Super-Resolution Fluorescence and Tomographic Electron Microscopy of Endocytic Proteins at the Nanometer Scale Kem A. Sochacki1, Gleb Shtengel2, Harald F. Hess2, Justin W. Taraska1. National Heart Lung and Blood Institute, Bethesda, MD, USA, HHMI: Janelia Farm, Ashburn, VA, USA. Three-dimensional super-resolution fluorescence microscopy methods such as interferometric photoactivated localization microscopy (iPALM) can localize individual proteins of interest at high resolution (20 nm in XY and 8 nm in Z). These images, however, lack the structural context that could be provided by other imaging methods including electron microscopy. Here, we develop a method that combines iPALM and 3D tomographic transmission electron microscopy (TEM). First, we use iPALM to localize fluorescently-tagged endocytic proteins on the inner plasma membrane of PC12 cells in three dimensions. Next, platinum replicas of these same membranes are imaged with transmission electron microscopy to create tomograms. Finally, both images are combined to create a map of the nanometer scale location of these proteins within their three dimensional cellular context. This technique has the power to build ultra high resolution 3D topographic maps of molecular structures in the context of their native cellular environment." @default.
• W3143643313 created "2021-04-13" @default.
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• W3143643313 date "2013-01-01" @default.
• W3143643313 modified "2023-09-23" @default.
• W3143643313 title "Optical Measurement of Biomechanical Properties of Human Red Blood Cell using Digital Holographic Microscopy: Malaria and Sickle Cell Diseases" @default.
• W3143643313 hasPublicationYear "2013" @default.
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