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- W3143853427 abstract "Objective To build the trestle of tissue engineering for skin with the collagen. Methods The collagen was obtained from the baby cattle hide pretreated by Na 2S and elastinase and Protease M, then the collagen was dissolved in 0.5 mol/L acetic acid solution. The collagen was treated with Protease N to minimize its immunogenicity. The resulting collagen could be used to build the trestle of tissue engineering for skin because of good biocompatibility. The collagen molecular weight and structure were analyzed by SDS PAGE. The bioactivity of trestle was tested in the experiment of the mice wound healing and the cell implantation. Results The SDS PAGE result of the collagen treated by Protease M showed the typical spectrum of type Ⅰ collagen. The built trestle was a collagen sponge matrix in which micropore size was 50 200μm. It could accelerate wound healing and the implanted fibroblasts could proliferate well. Conclusion The collagen treated by Protease N can get good biocompatibilily and is suitable for building the trestles of tissue engineering for skin with good bioactivity." @default.
- W3143853427 created "2021-04-13" @default.
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- W3143853427 date "2003-01-01" @default.
- W3143853427 modified "2023-09-23" @default.
- W3143853427 title "PREPARING TRESTLE OF TISSUE ENGINEERING FOR SKIN WITH COLLAGEN" @default.
- W3143853427 hasPublicationYear "2003" @default.
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