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- W3143981958 abstract "PREPARATION of HMW DNA (Megabase-size) is the basis for construction of genomic library with large DNA inserts such as bacterial artificial chromosome (BAC) and yeast artificial chromosome (YAC), and for long-range physical mapping. It can also be used for the macro-study of repeat sequences. Since HMW DNA during preparation is inclined to be sheared physically and digested by internal nucleases, it is very difficult to prepare the HMW DNA. Initially, plant HMW DNA was prepared by embedding protoplasts in the low melting-point (LMP) agarose; however, it had several disadvantages: (ⅰ) Culture of protoplasts was time-consuming, costly and tedious. ( ⅱ ) It was only used successfully for limited plant" @default.
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- W3143981958 date "1999-01-01" @default.
- W3143981958 modified "2023-09-26" @default.
- W3143981958 title "Rapid and efficient method for isolating plant high molecular weight(HMW)DNA with high purity" @default.
- W3143981958 hasPublicationYear "1999" @default.
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