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- W3144022914 abstract "目的 观察蓝光照射对人视网膜色素上皮（RPE）细胞L-型钙通道mRNA表达的影响。方法 体外培养的第4代人RPE细胞按随机数字表法分为无光照组、光照组、光照+硝苯地平组和光照+（-）BayK8644组。（2000plusmn;500）Lux蓝光照射6 h，终止细胞培养24 h。光照前加入硝苯地平和（-）BayK8644，浓度均为1times;10-5 mmol/L。荧光定量聚合酶链（PCR）技术检测各组人RPE细胞L-型钙通道alpha;1C亚型、alpha;1D亚型和alpha;1F亚型mRNA的表达。结果 荧光定量PCR产物alpha;1C、alpha;1D、alpha;1F和内参beta;-肌动蛋白的cDNA逆转录PCR扩增产物长度分别为68、157、125、186碱基对，产物的凝胶电泳结果显示扩增条带位置与以上片段相吻合。荧光定量PCR检测结果显示，光照组、光照+硝苯地平组、光照+(-)BayK8644组alpha;1C mRNA的表达均高于无光照组，差异有统计学意义(P＜0.05)；光照+硝苯地平组、光照+(-)BayK8644组alpha;1C mRNA的表达均高于光照组，差异有统计学意义(P＜0.05)；光照+(-)BayK8644组alpha;1C mRNA的表达高于光照+硝苯地平组，差异有统计学意义(P＜0.05)。光照组、光照+硝苯地平组、光照+(-)BayK8644组alpha;1D mRNA的表达均高于无光照组，差异均有统计学意义(P＜0.05)；光照+(-)BayK8644组alpha;1D mRNA的表达与光照组和光照+硝苯地平组比较，差异均有统计学意义(P＜0.05)；光照+硝苯地平组与光照组alpha;1D mRNA的表达比较，差异无统计学意义(P＞0.05）。光照组、光照+硝苯地平组、光照+(-)BayK8644组alpha;1F mRNA的表达均高于无光照组，差异有统计学意义(P＜0.05)；光照+硝苯地平组、光照+(-)BayK8644组alpha;1F mRNA的表达均高于光照组，差异有统计学意义(P＜0.05)。结论 蓝光照射可引起人RPE细胞L-型钙通道中alpha;1C、alpha;1D及alpha;1F亚型mRNA表达不同程度的增高；1times;10-5 mmol/L硝苯地平不能对抗蓝光对RPE细胞L-型钙通道作用，而1times;10-5 mmol/L（-）Bay K8644可以增加以上3个亚基的表达。" @default.
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- W3144022914 date "2016-09-02" @default.
- W3144022914 modified "2023-09-23" @default.
- W3144022914 title "蓝光照射对人视网膜色素上皮细胞L-型钙通道-mRNA表达的影响" @default.
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