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- W3144032106 abstract "Objective To establish a method of detecting and localizing newly cloned gene by FISH using short DNA fragment. Methods Bio 14 dATP was incorporated into the target gene cloned in plasmid by nick translation followed by conventional FISH with some modification. By Means of this method, c met, c erbB2, 1A6, APC and Rb genes were examined in ovarian and gastric cancer cell lines. Results 1A6, a newly isolated gene was located at the long arm of one of the C group chromosomes. Oncogene c met, c erbB2 and trmor suppressor gene APC, Rb were shown to produce clear cut hybridization signals in ovarian and gastric cancer cells. Conclusion Short DNA fragment cloned in plasmid is useful for studies of gene localization, gene deletion and amplification by FISH." @default.
- W3144032106 created "2021-04-13" @default.
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- W3144032106 date "1999-01-01" @default.
- W3144032106 modified "2023-09-23" @default.
- W3144032106 title "Gene detection and localization by FISH with short DNA fragment cloned in plasmid" @default.
- W3144032106 hasPublicationYear "1999" @default.
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