Matches in SemOpenAlex for { <https://semopenalex.org/work/W3144032886> ?p ?o ?g. }
- W3144032886 abstract "Background: Hepatitis C core protein is an attractive target for HCV vaccine aimed to exterminate HCV infected cells. However, although highly immunogenic in natural infection, core appears to have low immunogenicity in experimental settings. We aimed to design an HCV vaccine prototype based on core, and devise immunization regimens that would lead to potent anti-core immune responses which circumvent the immunogenicity limitations earlier observed. Methods: Plasmids encoding core with no translation initiation signal (pCMVcore); with Kozak sequence (pCMVcoreKozak); and with HCV IRES (pCMVcoreIRES) were designed and expressed in a variety of eukaryotic cells. Polyproteins corresponding to HCV 1b amino acids (aa) 1–98 and 1–173 were expressed in E. coli. C57BL/6 mice were immunized with four 25-g doses of pCMVcoreKozak, or pCMV (I). BALB/c mice were immunized with 100 g of either pCMVcore, or pCMVcoreKozak, or pCMVcoreIRES, or empty pCMV (II). Lastly, BALB/c mice were immunized with 20 g of core aa 1–98 in prime and boost, or with 100 g of pCMVcoreKozak in prime and 20 g of core aa 1–98 in boost (III). Antibody response, [3H]-T-incorporation, and cytokine secretion by core/core peptide-stimulated splenocytes were assessed after each immunization. Results: Plasmids differed in core-expression capacity: mouse fibroblasts transfected with pCMVcore, pCMVcoreIRES and pCMVcoreKozak expressed 0.22 ± 0.18, 0.83 ± 0.5, and 13 ± 5 ng core per cell, respectively. Single immunization with highly expressing pCMVcoreKozak induced specific IFN- and IL-2, and weak antibody response. Single immunization with plasmids directing low levels of core expression induced similar levels of cytokines, strong T-cell proliferation Published: 8 June 2009 Genetic Vaccines and Therapy 2009, 7:7 doi:10.1186/1479-0556-7-7 Received: 16 December 2008 Accepted: 8 June 2009 This article is available from: http://www.gvt-journal.com/content/7/1/7 © 2009 Alekseeva et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited." @default.
- W3144032886 created "2021-04-13" @default.
- W3144032886 creator A5005186173 @default.
- W3144032886 creator A5017506109 @default.
- W3144032886 creator A5019798570 @default.
- W3144032886 creator A5019913804 @default.
- W3144032886 creator A5038681512 @default.
- W3144032886 creator A5040763459 @default.
- W3144032886 creator A5043439939 @default.
- W3144032886 creator A5052120633 @default.
- W3144032886 creator A5063225070 @default.
- W3144032886 creator A5073929857 @default.
- W3144032886 creator A5078370672 @default.
- W3144032886 creator A5085351016 @default.
- W3144032886 date "2015-01-01" @default.
- W3144032886 modified "2023-10-18" @default.
- W3144032886 title "Genetic Vaccines and Therapy" @default.
- W3144032886 cites W118433064 @default.
- W3144032886 cites W1491528461 @default.
- W3144032886 cites W1526687826 @default.
- W3144032886 cites W1559533842 @default.
- W3144032886 cites W1560653090 @default.
- W3144032886 cites W156217820 @default.
- W3144032886 cites W165274422 @default.
- W3144032886 cites W1755605051 @default.
- W3144032886 cites W1872218686 @default.
- W3144032886 cites W1963902916 @default.
- W3144032886 cites W1964770087 @default.
- W3144032886 cites W1966270205 @default.
- W3144032886 cites W1968200256 @default.
- W3144032886 cites W1976177790 @default.
- W3144032886 cites W1977139084 @default.
- W3144032886 cites W1977779856 @default.
- W3144032886 cites W1979237882 @default.
- W3144032886 cites W1980826488 @default.
- W3144032886 cites W1982476312 @default.
- W3144032886 cites W1988670888 @default.
- W3144032886 cites W1995896311 @default.
- W3144032886 cites W1996564095 @default.
- W3144032886 cites W2002167432 @default.
- W3144032886 cites W2002319930 @default.
- W3144032886 cites W2005172972 @default.
- W3144032886 cites W2006757932 @default.
- W3144032886 cites W2008862517 @default.
- W3144032886 cites W2017359325 @default.
- W3144032886 cites W2018434707 @default.
- W3144032886 cites W2019746101 @default.
- W3144032886 cites W2026101939 @default.
- W3144032886 cites W2026449587 @default.
- W3144032886 cites W2029582445 @default.
- W3144032886 cites W2030331707 @default.
- W3144032886 cites W2030337163 @default.
- W3144032886 cites W2036027433 @default.
- W3144032886 cites W2037908741 @default.
- W3144032886 cites W2038355924 @default.
- W3144032886 cites W2039034666 @default.
- W3144032886 cites W2040448006 @default.
- W3144032886 cites W2045380565 @default.
- W3144032886 cites W2048047751 @default.
- W3144032886 cites W2048555465 @default.
- W3144032886 cites W2049746784 @default.
- W3144032886 cites W2053978445 @default.
- W3144032886 cites W2054334651 @default.
- W3144032886 cites W2054802322 @default.
- W3144032886 cites W2056416333 @default.
- W3144032886 cites W2056626388 @default.
- W3144032886 cites W2060272043 @default.
- W3144032886 cites W2060354832 @default.
- W3144032886 cites W2064807794 @default.
- W3144032886 cites W2066287861 @default.
- W3144032886 cites W2067415260 @default.
- W3144032886 cites W2068181941 @default.
- W3144032886 cites W2070426326 @default.
- W3144032886 cites W2070968956 @default.
- W3144032886 cites W2071267501 @default.
- W3144032886 cites W2074715052 @default.
- W3144032886 cites W2075077578 @default.
- W3144032886 cites W2075600964 @default.
- W3144032886 cites W2084186516 @default.
- W3144032886 cites W2085638170 @default.
- W3144032886 cites W2087064069 @default.
- W3144032886 cites W2087231599 @default.
- W3144032886 cites W2087736015 @default.
- W3144032886 cites W2088788596 @default.
- W3144032886 cites W2089167315 @default.
- W3144032886 cites W2091633336 @default.
- W3144032886 cites W2091663135 @default.
- W3144032886 cites W2093423705 @default.
- W3144032886 cites W2095135796 @default.
- W3144032886 cites W2097224767 @default.
- W3144032886 cites W2099199725 @default.
- W3144032886 cites W2100400849 @default.
- W3144032886 cites W2103742539 @default.
- W3144032886 cites W2115074165 @default.
- W3144032886 cites W2116017310 @default.
- W3144032886 cites W2118693878 @default.
- W3144032886 cites W2123511080 @default.
- W3144032886 cites W2126344726 @default.
- W3144032886 cites W2126795723 @default.
- W3144032886 cites W2131398155 @default.