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- W3144055487 abstract "The roles of the chemokine-like factor super family 1 and 2 ( CKLFSF 1 and CKLFSF 2) gene interval sequence was explored in transcriptional regulation. The target fragment was obtained by PCR amplification and inserted into pGL3 luciferase reporter vector. Using calcium phosphate precipitation mediated transient expression approach,the recombinant plasmids were transfected into HeLa cells respectively. The luciferase assay indicated that the luciferase activity was not detected when pGL3 Basic was transfected into HeLa cells. Nevertheless, the luciferase activity was markedly decreased when test plasmid was transfected into HeLa cells. The results suggested that the CKLFSF 1 and CKLFSF 2 gene interval sequence had no promoter activity, whereas some important cis acting silencer elements for the gene expression were localized within this sequence." @default.
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- W3144055487 date "2003-01-01" @default.
- W3144055487 modified "2023-09-27" @default.
- W3144055487 title "A Novel cis-element Exists Between CKLFSF1 and CKLFSF2 Genes" @default.
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