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- W3144059247 abstract "We have previously shown that the vasodilator effect of protopine (Pro) on rabbit aorta is related to the elevations of cAMP and cGMP. In the present study, the vasodilator mechanisms of Pro were further explored by recording the isotonic contraction of the rat aortic strips, detecting directly the intracellular free Ca2+ concentration ([Ca2+]i) with Fura-2/AM loaded vascular smooth muscle cells (VSMCs) of rat aorta, and determining the activity of protein kinase C (PKC) in rat aortic tissue with radioactive isotope γ-32P -ATP-catalyzing assay. By recording the aortic strips contraction induced by noradrenaline (NA) and high potassium (K+), Pro shifted nonparallelly the concentration-response curves of NA and high K+ to right, in which the maximal response was depressed in the presence of Pro (30 and 100 μmol/L), and the values of pD2' were 3.70?.25 and 3.97?.15 for NA and high K+, respectively. In the Fura-2/AM loaded VSMCs, Pro (50 and 100 μmol/L) could not produce any significant change on the resting [Ca2+]i, but significantly decreased the [Ca2+]i elevated by NA and high K+. Pro (30 and 100 μmol/L) had no significant effect on the activity of the cytosolic and membrane PKC in the aortic strips inpretreated by NA. However, in the aortic strips pretreated by NA, the activity of membrane PKC was significantly increased and the activity of cytosolic PKC tended to be decreased by Pro, while the activity of total PKC did not change. These results suggest that Pro seems to promote the translocation of PKC from the cytosol to the membrane in the presence of NA, its vasodilator effect may be the comprehensive result of its decreasing effect on the [Ca2+]. and the increasing effect on cAMP and cGMP, as well as its influence on the PKC." @default.
- W3144059247 created "2021-04-13" @default.
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- W3144059247 date "2005-01-01" @default.
- W3144059247 modified "2023-09-28" @default.
- W3144059247 title "Effects of protopine on intracellular calcium and the PKC activity of rat aorta smooth muscle" @default.
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