Matches in SemOpenAlex for { <https://semopenalex.org/work/W3144080376> ?p ?o ?g. }
- W3144080376 abstract "The Bombyx mori nucleopolyhedrovirus (BmNPV) is one of the most destructive diseases in silk- worm, which has caused the main damage to sericulture industry. In this study, we developed a system of RNAi to prevent the BmNPV infection using the piggyBac trans- poson-derived targeting short hairpin RNA (shRNA) interference. The shRNAs targeting the genes of i.e.-1, lef- 1, lef-2 and lef-3 of BmNPV were designed and used to inhibit the intracellular replication or multiplication of BmNPV in Bm cells. The highest activity was presented in the shRNA targeting the i.e.-1c of BmNPV, of which the inhibition rate reached 94.5 % in vitro. Further a stable Bm cell line of piggyBac transposon-derived targeting shRNA interference against BmNPV was established, which has a highly efficacious suppression on virus proliferation. These results indicated that the recombinant shRNA expression system was a useful tool for resistance to BmNPV in vitro. The approach by recombinant shRNAs opens a door of RNAi technology as a strategy that offering technically simpler, cheaper, and quicker gene knockdown for prom- ising research and biotechnology application on silkworm lethal diseases." @default.
- W3144080376 created "2021-04-13" @default.
- W3144080376 creator A5003875789 @default.
- W3144080376 creator A5062397887 @default.
- W3144080376 creator A5067964087 @default.
- W3144080376 creator A5070400348 @default.
- W3144080376 date "2014-01-01" @default.
- W3144080376 modified "2023-09-27" @default.
- W3144080376 title "piggyBac transposon-derived targeting shRNA interference against the Bombyx mori nucleopolyhedrovirus (BmNPV)" @default.
- W3144080376 cites W1498885215 @default.
- W3144080376 cites W1518586105 @default.
- W3144080376 cites W1524399376 @default.
- W3144080376 cites W1524545767 @default.
- W3144080376 cites W1552414630 @default.
- W3144080376 cites W1565205712 @default.
- W3144080376 cites W1611419331 @default.
- W3144080376 cites W1660934004 @default.
- W3144080376 cites W1689351977 @default.
- W3144080376 cites W1726937916 @default.
- W3144080376 cites W1788275706 @default.
- W3144080376 cites W1845902827 @default.
- W3144080376 cites W1860707285 @default.
- W3144080376 cites W1877936053 @default.
- W3144080376 cites W1886075534 @default.
- W3144080376 cites W1967219354 @default.
- W3144080376 cites W2012647813 @default.
- W3144080376 cites W2012771733 @default.
- W3144080376 cites W2023257972 @default.
- W3144080376 cites W2033067673 @default.
- W3144080376 cites W2034787548 @default.
- W3144080376 cites W2038783205 @default.
- W3144080376 cites W2040701123 @default.
- W3144080376 cites W2043368732 @default.
- W3144080376 cites W2044363259 @default.
- W3144080376 cites W2050419270 @default.
- W3144080376 cites W2080871194 @default.
- W3144080376 cites W2094730681 @default.
- W3144080376 cites W2099308992 @default.
- W3144080376 cites W2099333600 @default.
- W3144080376 cites W2107277218 @default.
- W3144080376 cites W2110137662 @default.
- W3144080376 cites W2112331345 @default.
- W3144080376 cites W2117855143 @default.
- W3144080376 cites W2123372646 @default.
- W3144080376 cites W2134108862 @default.
- W3144080376 cites W2139196607 @default.
- W3144080376 cites W2157594098 @default.
- W3144080376 cites W2160775002 @default.
- W3144080376 cites W2163428263 @default.
- W3144080376 cites W2468468885 @default.
- W3144080376 cites W3748054 @default.
- W3144080376 hasPublicationYear "2014" @default.
- W3144080376 type Work @default.
- W3144080376 sameAs 3144080376 @default.
- W3144080376 citedByCount "0" @default.
- W3144080376 crossrefType "journal-article" @default.
- W3144080376 hasAuthorship W3144080376A5003875789 @default.
- W3144080376 hasAuthorship W3144080376A5062397887 @default.
- W3144080376 hasAuthorship W3144080376A5067964087 @default.
- W3144080376 hasAuthorship W3144080376A5070400348 @default.
- W3144080376 hasConcept C102230213 @default.
- W3144080376 hasConcept C104317684 @default.
- W3144080376 hasConcept C119056186 @default.
- W3144080376 hasConcept C136961756 @default.
- W3144080376 hasConcept C143065580 @default.
- W3144080376 hasConcept C153911025 @default.
- W3144080376 hasConcept C159047783 @default.
- W3144080376 hasConcept C166703698 @default.
- W3144080376 hasConcept C173396325 @default.
- W3144080376 hasConcept C190232843 @default.
- W3144080376 hasConcept C203233990 @default.
- W3144080376 hasConcept C2522874641 @default.
- W3144080376 hasConcept C4918238 @default.
- W3144080376 hasConcept C54355233 @default.
- W3144080376 hasConcept C67705224 @default.
- W3144080376 hasConcept C86803240 @default.
- W3144080376 hasConcept C95444343 @default.
- W3144080376 hasConceptScore W3144080376C102230213 @default.
- W3144080376 hasConceptScore W3144080376C104317684 @default.
- W3144080376 hasConceptScore W3144080376C119056186 @default.
- W3144080376 hasConceptScore W3144080376C136961756 @default.
- W3144080376 hasConceptScore W3144080376C143065580 @default.
- W3144080376 hasConceptScore W3144080376C153911025 @default.
- W3144080376 hasConceptScore W3144080376C159047783 @default.
- W3144080376 hasConceptScore W3144080376C166703698 @default.
- W3144080376 hasConceptScore W3144080376C173396325 @default.
- W3144080376 hasConceptScore W3144080376C190232843 @default.
- W3144080376 hasConceptScore W3144080376C203233990 @default.
- W3144080376 hasConceptScore W3144080376C2522874641 @default.
- W3144080376 hasConceptScore W3144080376C4918238 @default.
- W3144080376 hasConceptScore W3144080376C54355233 @default.
- W3144080376 hasConceptScore W3144080376C67705224 @default.
- W3144080376 hasConceptScore W3144080376C86803240 @default.
- W3144080376 hasConceptScore W3144080376C95444343 @default.
- W3144080376 hasLocation W31440803761 @default.
- W3144080376 hasOpenAccess W3144080376 @default.
- W3144080376 hasPrimaryLocation W31440803761 @default.
- W3144080376 hasRelatedWork W1558910933 @default.
- W3144080376 hasRelatedWork W1981390890 @default.
- W3144080376 hasRelatedWork W1987682150 @default.