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- W3144172347 abstract "AIM To establish a RP HPLC method for determination of cyclovirobuxine D. METHODS Cyclovirobuxine D reacted with a derivative reagent 1 naphthyl isocyanate in ch loroform to form fluorescence derivatives, stopped the reaction by adding the mo bile phase and then directly injected the solution into the chromatograph to sep erate it by RP HPLC. The analysis was carried out on C 18 column, the mobi le phase is methanol water (85∶15), the excitation wavelength was set at 305 n m, emission at wavelength 385 nm, and the flow rate was 1 mL·min -1 . T he effect of several factors including the reaction medium, temperature, time an d amount of 1 naphthyl isocyanate on the yield of the derivatization was also i nvestigated systematically. RESULTS A simple and rapid RP HPLC method for the simultaneous isolation and analysis o f cyclovirobuxine D and its related substances was developed, and the absence of interference between the derivative peak responses of cyclovirobuxine D and its related substances were verified by UV diode array detecter and MS. The lineari ty was obtained from 0 75 μg·mL -1 to 2 5 μg·mL -1 of cy clovirobuxine D derivatives with a correlation coefficient of 0 9991. The detec tion limit of cyclovirobuxine D derivative was 1 ng·mL -1 , the repeata bility of derivatization was good with relative standard derivation no more than 1 2% and derivative was stable within 48 h. The method described conforms to t he validation of China Pharmacopiea compendial methods used for pharmaceutical p roducts in general. CONCLUSION The established method is proved to be reliable quantitative method for the qual ity control of cyclovirobuxine D." @default.
- W3144172347 created "2021-04-13" @default.
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- W3144172347 date "2002-01-01" @default.
- W3144172347 modified "2023-09-25" @default.
- W3144172347 title "DETERMINATION OF CYCLOVIROBUXINED BY RP-HPLC WITH PRECOLUMN FLUORESCENCE DERIVATIZATION" @default.
- W3144172347 hasPublicationYear "2002" @default.
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