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- W3144328109 abstract "[Objectives]This study sought to assess mitochondrial DNA( mt DNA) diversity and origin of Daweishan Mini Chicken from Yunnan Province. [Methods]Blood samples from a total of 30 individuals belonging to Daweishan Mini breed were collected.The complete of the D-loop was PCR amplified and subsequently sequenced. DNA sequence data of five red Jungle fowl species were also collected from the Gen Bank for use as the reference sequences. The date analysis ways of biologic informatics were used.[Results]The results showed that,the D-loop region of Daweishan Mini Chicken was 1 231-1 232 bp,and the 1 231 bp haplotype had a base C deficiency within 859 bp site. The average coefficient of nucleotide variation was 5.448,the nucleotide diversity( Pi)was 0.004 43±0.000 14,and the haplotype diversity( Hd) was 0.685± 0.079. Eighteen variable sites that defined 6 haplotypes were observed. The 6 haplotypes clustered into four clades( clade A,B,D,and E),of which clade A and B were dominant. Two haplotypes belong to clade A,two haplotypes are in clade B,one is in clade D,and one haploype is in clade E. The clade A and clade B were clustered with Gallus gallus spadiceus. [Conclusions]These results show a high mitochondrial D-loop diversity and indicate multiple maternal origins for Daweishan Mini Chickens. Gallus gallus spadiceus contributed more to the forming process." @default.
- W3144328109 created "2021-04-13" @default.
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- W3144328109 date "2015-01-01" @default.
- W3144328109 modified "2023-09-28" @default.
- W3144328109 title "The investigation of genetic diversity and evolution of Daweishan Mini Chicken based on complete mitochondrial DNA D-loop region sequence" @default.
- W3144328109 hasPublicationYear "2015" @default.
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