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- W3144642988 abstract "Objective To analyze the status of human metapneumovirus(hMPV) in wheezing children in East Guangdong and the clinical characteristics of the diseases with hMPV infection.Methods The viral nucleic acid of 323 samples of nasopharyngeal secretions,throat,or tracheal swabs were amplified by RT-PCR(240 from children with wheezing,83 from health children),and six hMPV N gene positive PCR products were randomly chosen for purified and sequenced.Nucleotide sequences alignment and phylogenetic analysis were performed with DNAstar software.The clinical data of the children with hMPV infection were analyzed.Results The positive rate of hMPV infection identified from 240 children with wheezing was 5%(12/240).hMPV was significantly more prevalent in March and April.All the positive cases aged14 months.Six hMPV N gene fragments of sequence were closely related to hMPV in the GenBank.Similarity of hMPV partial N gene with hMPV N gene published at nucleotide levels varied from 80.8% to 98.4%.Phylogenetic tree analysis of nucleotide of nucleotide sequences revealed two different genotypes.All hMPV-posotive patients suffered from fever,cough and wheezing.The clinical diagnoses of hMPV-posotive patients were wheezy pneumonia (four patients),bronchiolitis(eight patients).None of the nasal swabs from 83 healthy subjects were tested hMPV-specific gene fragment.Conclusions hMPV is an important viral pathogen in children with wheezing in East Guangdong.hMPVs of different genotypes may co-circulate in children in East Guangdong." @default.
- W3144642988 created "2021-04-13" @default.
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- W3144642988 date "2009-01-01" @default.
- W3144642988 modified "2023-09-26" @default.
- W3144642988 title "Identified and etiological study on human metapneumovirus in children with wheezing in East Guangdong" @default.
- W3144642988 hasPublicationYear "2009" @default.
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