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• W3144802398 abstract "CD44v6 is a cancer-associated antigen that mainly expresses in a subset of adenocarcinomas. Therefore, in this study, anti-human CD44v6 single-chain variable fragment (scFv) has been selected and characterized because it is the first step of primary importance towards the con- struction of a novel cancer-targeted agent for cancer diag- nosis and therapy. In our study, anti-human CD44v6 scFv was selected from a human phage-displayed scFv library based on its ability to bind in vitro to CD44v6 antigen. Subsequently, immunofluorescent staining and Western blot analyses were performed to measure the binding character- istics of this scFv. In addition, flow cytometric analysis was done to verify its cancer-targeting ability in vitro. And a flow cytometry-based assay was used to determine its equilib- rium dissociation constant (KD). Finally, one functional anti- CD44v6 scFv was selected and characterized. Nucleotide sequencing verified that it was an incomplete scFv gene but had a variable heavy chain (VH) alone. However, anti- CD44v6 scFv demonstrated cell-binding and antigen- binding activities by immunofluorescent staining and Wes- tern blot analyses. Furthermore, flow cytometric analysis proved that this scFv specifically targeted CD44v6- expressing cancer cells other than CD44v6 non-expressing normal cells or tumor cells in vitro. The KD of this scFv was calculated to be 7.85 ± 0.93 9 10 -8 M. In summary, the selected human scFv against CD44v6 has specific binding activity and favorable binding affinity despite lacking a variable light chain (VL). Moreover, it can effectively and specifically target CD44v6-expressing cancer cells. All these characteristics make anti-CD44v6 scFv a promising agent for cancer detection and anti-cancer therapy." @default.
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• W3144802398 date "2010-01-01" @default.
• W3144802398 modified "2023-09-27" @default.
• W3144802398 title "Generation of a stable anti-human CD44v6 scFv and analysis of its cancer-targeting ability in vitro" @default.
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