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- W3144863860 abstract "Objective To investigate the effect of BMP-2 on (the) gene expression of rat osteosarcoma cells (ROS17/2.8) under rotating clinostat simulated weightlessness. Mothod ROS17/2.8 cells were cultured in 1 G control and rotating clinostat simulated weightlessness with 500 ng/ml BMP-2 in the culture medium. Total RNA in cells was isolated after 24, 48 and 72 h. Reverse transcription PCR analysis was made to examine the gene expression of α1 chain of type I collagen (collagen Ⅰ α1) and alkaline phosphatase (ALP). Result The expression of COL-Ⅰα1 mRNA induced by BMP-2 was much more than that without BMP-2 in 24 h and 48 h group(P0.01). The level of ALP mRNA induced by BMP-2 was significantly high in 48 h or 72 h group(respectively P 0.01,P 0.05). The level of BMP-2 induced expression of COL-Ⅰα1 mRNA was significantly lower under 48 h of simulated weightlessness than in 1G condition( P0.01). The level of ALP mRNA was significantly lower under simulated weightlessness for 48 h or 72 h(P0.01). Conclusion BMP-2 can stimulate the differentiation of ROS17/2.8 cells in 1 G condition and this process is reduced under simulated weightlessness." @default.
- W3144863860 created "2021-04-13" @default.
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- W3144863860 date "2004-01-01" @default.
- W3144863860 modified "2023-09-23" @default.
- W3144863860 title "Effects of BMP-2 on the Gene Expression of Rat Osteosarcoma Cells under Simulated Weightlessness." @default.
- W3144863860 hasPublicationYear "2004" @default.
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