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- W3144868685 abstract "Proctective antigen gene FH3 of F. hepatica was cut out from recombinant plasmid pUC18/FH3 using EcoRI and then cloned into a eukaryotic expression vector pBlueCMV,and a F. hepatica DNA vaccine pBlueCMV-FH3 was constructed. After prelation and purification, the DNA vaccine was transferred into Kunming mice in the amount of 100ug/mouse through intramuscular inoculation. Muscle tissues of some mice were cut off to test the expression of FH3 antigen, and the result showed that FH3 antigen was indeed expressed. The humoral immune response results showed that this vaccine could induce specific antibodies,Furthermore, the titers of mice immuned 3 times were much higher than those of immuned single time. After immuned 3 times, some mice were challenged with cysticercus (20 cysticercus per mouse) of F. hepatica,and the immuno-protection rate was 41%." @default.
- W3144868685 created "2021-04-13" @default.
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- W3144868685 date "2000-01-01" @default.
- W3144868685 modified "2023-09-25" @default.
- W3144868685 title "CONSTRUCTION A DNA VACCINE OF FASCIOLA.HEPATICA AND THE PRIMARY STUDY ON ITS IMMUNE RESPONSES" @default.
- W3144868685 hasPublicationYear "2000" @default.
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