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- W3144927137 abstract "To explore the effect of SOS response on enrofloxacin resistance in E. coli, we deleted the rec A gene, which encodes the inducer of SOS response, and evaluated the combined inhibitory effect of Rec A inhibitor and enrofloxacin on E. coli in vitro. By Red recombination, a deficient strain E. coli ATCC 25922/△rec A was obtained. The expression of rec A and umu C under the pressure of enrofloxacin was measured by real-time PCR. The minimum inhibitory concentration(MIC) of E. coli ATCC 25922 and E. coli ATCC 25922/△rec A to many kinds of antibiotics, including enrofloxacin, were detected by broth dilution method. And the effect of rec A deficiency on resistance variant to enrofloxacin was investigated by gradient plate method. N6-(1-naphthyl)-ADP, the inhibitor of SOS response, was synthesized and its influence combined with enrofloxacin on bacterial growth and resistance was evaluated. As results, E. coli ATCC 25922/△rec A was established successfully and its MIC was decreased to an eighth of original strain. Moreover, the rec A deficient strain was less prone to develop resistance than E. coli ATCC 25922 when exposed to enrofloxacin. The results of real-time PCR showed that SOS response was inhibited in rec A-deleted strain or with the action of Rec A inhibitor. The rec A-deleted E. coli ATCC 25922 was more susceptive to enrofloxacin and the rate of resistant variation was lower than that in wild-type strain, and the inhibitor of Rec A can inhibit SOS response to a certain degree and assist enrofloxacin to inhibit bacteria growth." @default.
- W3144927137 created "2021-04-13" @default.
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- W3144927137 date "2015-01-01" @default.
- W3144927137 modified "2023-09-24" @default.
- W3144927137 title "Effect of SOS-response Inhibition on Enrofloxacin Resistance in Escherichia coli" @default.
- W3144927137 hasPublicationYear "2015" @default.
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