Matches in SemOpenAlex for { <https://semopenalex.org/work/W3145087164> ?p ?o ?g. }
Showing items 1 to 83 of
83
with 100 items per page.
- W3145087164 endingPage "572" @default.
- W3145087164 startingPage "565" @default.
- W3145087164 abstract "Objective Endothelial apoptosis plays an important role in the initiation of atherosclerosis. It would be useful to clarify whether activation of non-neuronal muscarinic receptor(NNMR) could prevent endothelial apoptosis and atherosclerosis. We investigated the effects of NNMR activation on regulating rat aortic endothelial cells(RAECs) apoptosis induced by homocysteine, an independent risk factor of atherosclerosis, and further studied its molecular mechanism. Methods RAECs were incubated using homocysteine at the concentration of 2.7 mmol/L for 36 h. RAECs were also pre-treated with carbachol or arecoline to examine their effects. RT-PCR was used to assess changes in the gene expression related to cell apoptosis. Results Incubation of RAECs with homocysteine at the concentration of 2.7 mmol/L resulted in morphologic changes, such as cellular shrinkage, membrane blebbing, chromatin condensation and margination. These could be attenuated by pretreatment with carbachol and arecoline at the concentration of 10 μmol/L for 12 h. Homocysteine induced apoptosis in RAECs and the molecular mechanisms were associated with the regulation of fas, fas-L and caspase-8 in the death receptor pathway, bcl-2, bcl-xL and bax in the mitochondrial pathway, caspase-12 in the endoplasmic reticulum pathway and caspase-3, caspase-6 and p53 as downstream effectors. Carbachol and arecoline attenuated the effects of homocysteine on genes in the death receptor pathway, in the mitochondrial pathway and in the downstream pathway. Atropine could reverse all of the effects of arecoline. Conclusion Activation of NNMR by carbacol and arecoline inhibits homocysteine-induced endothelial cell apoptosis mainly through regulation of death receptor pathway, mitochondrial pathway and downstream effectors." @default.
- W3145087164 created "2021-04-13" @default.
- W3145087164 creator A5001978013 @default.
- W3145087164 creator A5008760776 @default.
- W3145087164 creator A5020585088 @default.
- W3145087164 creator A5028515700 @default.
- W3145087164 creator A5032245741 @default.
- W3145087164 creator A5053780153 @default.
- W3145087164 creator A5085545053 @default.
- W3145087164 creator A5089121389 @default.
- W3145087164 creator A5089182802 @default.
- W3145087164 creator A5090599970 @default.
- W3145087164 date "2013-01-01" @default.
- W3145087164 modified "2023-09-23" @default.
- W3145087164 title "Non-neuronal muscarinic receptor activation prevents apoptosis of endothelial cells induced by homocysteine" @default.
- W3145087164 hasPublicationYear "2013" @default.
- W3145087164 type Work @default.
- W3145087164 sameAs 3145087164 @default.
- W3145087164 citedByCount "0" @default.
- W3145087164 crossrefType "journal-article" @default.
- W3145087164 hasAuthorship W3145087164A5001978013 @default.
- W3145087164 hasAuthorship W3145087164A5008760776 @default.
- W3145087164 hasAuthorship W3145087164A5020585088 @default.
- W3145087164 hasAuthorship W3145087164A5028515700 @default.
- W3145087164 hasAuthorship W3145087164A5032245741 @default.
- W3145087164 hasAuthorship W3145087164A5053780153 @default.
- W3145087164 hasAuthorship W3145087164A5085545053 @default.
- W3145087164 hasAuthorship W3145087164A5089121389 @default.
- W3145087164 hasAuthorship W3145087164A5089182802 @default.
- W3145087164 hasAuthorship W3145087164A5090599970 @default.
- W3145087164 hasConcept C170493617 @default.
- W3145087164 hasConcept C185592680 @default.
- W3145087164 hasConcept C190283241 @default.
- W3145087164 hasConcept C2776789625 @default.
- W3145087164 hasConcept C2778988552 @default.
- W3145087164 hasConcept C31573885 @default.
- W3145087164 hasConcept C33789571 @default.
- W3145087164 hasConcept C55493867 @default.
- W3145087164 hasConcept C86803240 @default.
- W3145087164 hasConcept C95444343 @default.
- W3145087164 hasConcept C98424977 @default.
- W3145087164 hasConceptScore W3145087164C170493617 @default.
- W3145087164 hasConceptScore W3145087164C185592680 @default.
- W3145087164 hasConceptScore W3145087164C190283241 @default.
- W3145087164 hasConceptScore W3145087164C2776789625 @default.
- W3145087164 hasConceptScore W3145087164C2778988552 @default.
- W3145087164 hasConceptScore W3145087164C31573885 @default.
- W3145087164 hasConceptScore W3145087164C33789571 @default.
- W3145087164 hasConceptScore W3145087164C55493867 @default.
- W3145087164 hasConceptScore W3145087164C86803240 @default.
- W3145087164 hasConceptScore W3145087164C95444343 @default.
- W3145087164 hasConceptScore W3145087164C98424977 @default.
- W3145087164 hasIssue "6" @default.
- W3145087164 hasLocation W31450871641 @default.
- W3145087164 hasOpenAccess W3145087164 @default.
- W3145087164 hasPrimaryLocation W31450871641 @default.
- W3145087164 hasRelatedWork W151714596 @default.
- W3145087164 hasRelatedWork W2014727273 @default.
- W3145087164 hasRelatedWork W2040492249 @default.
- W3145087164 hasRelatedWork W2065965523 @default.
- W3145087164 hasRelatedWork W2086070603 @default.
- W3145087164 hasRelatedWork W2138532578 @default.
- W3145087164 hasRelatedWork W2313518958 @default.
- W3145087164 hasRelatedWork W2322005335 @default.
- W3145087164 hasRelatedWork W2350111599 @default.
- W3145087164 hasRelatedWork W2526189678 @default.
- W3145087164 hasRelatedWork W2568728530 @default.
- W3145087164 hasRelatedWork W2620616004 @default.
- W3145087164 hasRelatedWork W2886973577 @default.
- W3145087164 hasRelatedWork W2921681372 @default.
- W3145087164 hasRelatedWork W3037731668 @default.
- W3145087164 hasRelatedWork W3119930094 @default.
- W3145087164 hasRelatedWork W3127532080 @default.
- W3145087164 hasRelatedWork W3143334165 @default.
- W3145087164 hasRelatedWork W3152009423 @default.
- W3145087164 hasRelatedWork W49526896 @default.
- W3145087164 hasVolume "29" @default.
- W3145087164 isParatext "false" @default.
- W3145087164 isRetracted "false" @default.
- W3145087164 magId "3145087164" @default.
- W3145087164 workType "article" @default.