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- W3145264877 abstract "Using electronic cloning method,the putative disease resistance protein RGA4 and the homologue of the potato Rpi-blb1 protein were obtained from tomato's protein sequences database in NCBI by protein-blast when the sequence of late blight resistant protein Rpi-blb1 from potato was used as query sequence,then,they were compared in gene mapping,gene structure,modify,secondary structure,topological structure and electronic gene expression profile,etc,for proving their evolutionary relationship. The result showed that tomato RGA4 protein( XP_004245923. 1) was composed of988 amino acid residues with NB-ARC and LRR8 conserved domain,the corresponding c DNA sequence( XM-004245875. 1) was located on SL2. 40 region in the chromosome 8,with genomic DNA length 4 089 bp,the alignment region in chromosome 8 between 57228847- 57232935 bp,and composed of two exons and one intron. The tomato RGA4 protein was an unstable globular secretory protein without transmembrane structure. Tomato RGA4 protein was regarded as an orthologous of potato Rpi-blb1 protein because they were similar in secondary structure composition,gene sequence composition,gene mapping,etc,but their minor differences in gene evolution and composition could result in different functions. This study would provide theory for cloning late blight resistant gene and its utilization in tomato breeding of disease resistance." @default.
- W3145264877 created "2021-04-13" @default.
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- W3145264877 date "2015-01-01" @default.
- W3145264877 modified "2023-09-28" @default.
- W3145264877 title "Homology analysis of tomato RGA4 and potato Rpi-blb1 protein" @default.
- W3145264877 hasPublicationYear "2015" @default.
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