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- W3145420 abstract "The polyglutamine diseases are caused by the expansion of CAG repeats. A key step in understanding the disease mechanisms, at the DNA and protein level, is the ability to produce recombinant proteins with specific length glutamine tracts which is a time-consuming first step in setting up in vitro systems to study the effects of polyglutamine expansion. Described here is a PCR-based method for the amplification of CAG repeats, which we used to incrementally extend CAG length by 3-5 repeats per cycle. This method could be translated into various contexts where amplification of repeating elements is necessary." @default.
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- W3145420 date "2013-01-01" @default.
- W3145420 modified "2023-09-27" @default.
- W3145420 title "A Method for the Incremental Expansion of Polyglutamine Repeats in Recombinant Proteins" @default.
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- W3145420 doi "https://doi.org/10.1007/978-1-62703-438-8_5" @default.
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