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- W3145424263 abstract "Objective To evaluate the effects of mechanical stress on the formation and expression of core binding factor α1(Cbfα1) in MG-63 cells cultured on titanium in vitro. Methods MG-63 cells cultured on the titanium were subjected to a centrifugal force(2.205 N) 15 min per 4 hours and collected after 4, 8 and 12 hours. The formation and expression of Cbfα1 were examined by immunofluorescence staining and reverse transcription-polymerase chain reaction(RT-PCR). Results Both the cells with or without centrifugal force created the fluorescence in the nucleus and the immunofluorescence intensity of Cbfα1 in MG-63 cells with centrifugal force were higher than those without centrifugal force(P0.05). Meanwhile, both the cells with or without centrifugal force expressed the mRNA of Cbfα1 and the relative mRNA level of Cbfα1 in MG -63 cells with centrifugal force were higher than those without centrifugal force, and the differences were great significan(tP0.05). Conclusion Mechanical stress are beneficial to the formation and expression of Cbfα1." @default.
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- W3145424263 date "2008-01-01" @default.
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- W3145424263 title "Effects of mechanical stress on the formation and expression of Cbfα1 in vitro" @default.
- W3145424263 hasPublicationYear "2008" @default.
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