FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3145568800> ?p ?o ?g. }

• W3145568800 endingPage "812" @default.
• W3145568800 startingPage "803" @default.
• W3145568800 abstract "Background and Aim: The present serodiagnosis of brucellosis in livestock is based on the whole cell or smooth lipopolysaccharide of the Brucella organism in which specificity is hampered by the cross-reactivity, especially with the antibodies against Yersinia enterocolitica O:9 organism. The problem can be addressed by screening for better immunodominant antigens. Hence, the present study was undertaken to screen protein antigens of Brucella abortus for their diagnostic potential in cattle brucellosis. Materials and Methods: Protein antigens of B. abortus (n=10) non-reactive to antibodies against Y. enterocolitica O:9 were selected, expressed in Escherichia coli, assessed the reactivity of expressed recombinant proteins by Western blot, standardized indirect-enzyme-linked immunosorbent assay (ELISA) for detecting Brucella antibodies in cattle serum, and comparative evaluation was done. Results: All the selected protein antigens were expressed and in the Western blot with Brucella antibodies positive cattle serum, six recombinant (Brucella protein 26 [BP26], Cu-Zn Superoxide dismutase [SodC], B. abortus I-1885, Serine protease, Bacterioferritin, and Brucella Lumazine Synthase [BLS]) proteins showed reaction whereas none of the proteins showed reactivity with Brucella negative cattle serum. ELISA has been done using known Brucella positive and negative cattle sera samples (n=113 each) in which the performance of recombinant proteins in diagnosing brucellosis was in the order of BP26 > BLS > SodC followed by rest of the proteins. BP26 based ELISA was found to be better with area under the curve as 0.953, and diagnostic sensitivity, diagnostic specificity, and Youden's index of 90.27%, 95.58%, and 0.8584, respectively, with the excellent agreement (k=0.85). Conclusion: BP26 could be a potential diagnostic antigen among the immunodominant proteins of B. abortus in ruling out Y. enterocolitica O:9 infection while diagnosing brucellosis in cattle herds." @default.
• W3145568800 created "2021-04-13" @default.
• W3145568800 creator A5010666269 @default.
• W3145568800 creator A5015254403 @default.
• W3145568800 creator A5018306957 @default.
• W3145568800 creator A5022355086 @default.
• W3145568800 creator A5033360347 @default.
• W3145568800 creator A5050096369 @default.
• W3145568800 creator A5059764679 @default.
• W3145568800 creator A5061114416 @default.
• W3145568800 creator A5077454819 @default.
• W3145568800 creator A5079303451 @default.
• W3145568800 creator A5086260685 @default.
• W3145568800 date "2021-03-30" @default.
• W3145568800 modified "2023-09-23" @default.
• W3145568800 title "Comparative evaluation of the immunodominant proteins of Brucella abortus for the diagnosis of cattle brucellosis" @default.
• W3145568800 cites W1967856030 @default.
• W3145568800 cites W1975230963 @default.
• W3145568800 cites W1987737132 @default.
• W3145568800 cites W2042683431 @default.
• W3145568800 cites W2050457526 @default.
• W3145568800 cites W2051265367 @default.
• W3145568800 cites W2053073180 @default.
• W3145568800 cites W2058095769 @default.
• W3145568800 cites W2062441550 @default.
• W3145568800 cites W2113685276 @default.
• W3145568800 cites W2120919992 @default.
• W3145568800 cites W2148154323 @default.
• W3145568800 cites W2156638196 @default.
• W3145568800 cites W2161008197 @default.
• W3145568800 cites W2173310076 @default.
• W3145568800 cites W2270974465 @default.
• W3145568800 cites W2412050599 @default.
• W3145568800 cites W2567193360 @default.
• W3145568800 cites W2588605929 @default.
• W3145568800 cites W4210504714 @default.
• W3145568800 cites W4232681009 @default.
• W3145568800 cites W4243708085 @default.
• W3145568800 cites W4296148531 @default.
• W3145568800 doi "https://doi.org/10.14202/vetworld.2021.803-812" @default.
• W3145568800 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8076471" @default.
• W3145568800 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33935431" @default.
• W3145568800 hasPublicationYear "2021" @default.
• W3145568800 type Work @default.
• W3145568800 sameAs 3145568800 @default.
• W3145568800 citedByCount "4" @default.
• W3145568800 countsByYear W31455688002021 @default.
• W3145568800 countsByYear W31455688002022 @default.
• W3145568800 countsByYear W31455688002023 @default.
• W3145568800 crossrefType "journal-article" @default.
• W3145568800 hasAuthorship W3145568800A5010666269 @default.
• W3145568800 hasAuthorship W3145568800A5015254403 @default.
• W3145568800 hasAuthorship W3145568800A5018306957 @default.
• W3145568800 hasAuthorship W3145568800A5022355086 @default.
• W3145568800 hasAuthorship W3145568800A5033360347 @default.
• W3145568800 hasAuthorship W3145568800A5050096369 @default.
• W3145568800 hasAuthorship W3145568800A5059764679 @default.
• W3145568800 hasAuthorship W3145568800A5061114416 @default.
• W3145568800 hasAuthorship W3145568800A5077454819 @default.
• W3145568800 hasAuthorship W3145568800A5079303451 @default.
• W3145568800 hasAuthorship W3145568800A5086260685 @default.
• W3145568800 hasBestOaLocation W31455688001 @default.
• W3145568800 hasConcept C104317684 @default.
• W3145568800 hasConcept C147483822 @default.
• W3145568800 hasConcept C159047783 @default.
• W3145568800 hasConcept C159654299 @default.
• W3145568800 hasConcept C203014093 @default.
• W3145568800 hasConcept C2776234502 @default.
• W3145568800 hasConcept C2776415932 @default.
• W3145568800 hasConcept C2777692091 @default.
• W3145568800 hasConcept C2780767784 @default.
• W3145568800 hasConcept C2781028839 @default.
• W3145568800 hasConcept C2781429187 @default.
• W3145568800 hasConcept C40767141 @default.
• W3145568800 hasConcept C523546767 @default.
• W3145568800 hasConcept C54355233 @default.
• W3145568800 hasConcept C55493867 @default.
• W3145568800 hasConcept C86803240 @default.
• W3145568800 hasConcept C89423630 @default.
• W3145568800 hasConceptScore W3145568800C104317684 @default.
• W3145568800 hasConceptScore W3145568800C147483822 @default.
• W3145568800 hasConceptScore W3145568800C159047783 @default.
• W3145568800 hasConceptScore W3145568800C159654299 @default.
• W3145568800 hasConceptScore W3145568800C203014093 @default.
• W3145568800 hasConceptScore W3145568800C2776234502 @default.
• W3145568800 hasConceptScore W3145568800C2776415932 @default.
• W3145568800 hasConceptScore W3145568800C2777692091 @default.
• W3145568800 hasConceptScore W3145568800C2780767784 @default.
• W3145568800 hasConceptScore W3145568800C2781028839 @default.
• W3145568800 hasConceptScore W3145568800C2781429187 @default.
• W3145568800 hasConceptScore W3145568800C40767141 @default.
• W3145568800 hasConceptScore W3145568800C523546767 @default.
• W3145568800 hasConceptScore W3145568800C54355233 @default.
• W3145568800 hasConceptScore W3145568800C55493867 @default.
• W3145568800 hasConceptScore W3145568800C86803240 @default.
• W3145568800 hasConceptScore W3145568800C89423630 @default.
• W3145568800 hasIssue "3" @default.
• W3145568800 hasLocation W31455688001 @default.

• next