Matches in SemOpenAlex for { <https://semopenalex.org/work/W3145660107> ?p ?o ?g. }
Showing items 1 to 100 of
100
with 100 items per page.
- W3145660107 abstract "In this study, we report the development of a simple and efficient system for genetic transformation of the medicinal fungus Ganoderma lucidum. Various parameters were optimized to obtain successful Agrobac- terium tumefaciens-mediated transformation. Co-cultiva- tion of bacteria and protoplast at a ratio of 1,000:1 at 25C in medium containing 0.2 mM acetosyringone was found to be the optimum condition for high efficiency transfor- mation. Four plasmids, each carrying a different promoter driving the expression of an antibiotic resistance marker, were tested. The construct carrying the Ganoderma luci- dum glyceraldehyde-3-phosphate dehydrogenase (GPD) promoter showed good transformation efficiency, whereas constructs with the GPD promoter from ascomycetes were ineffective. Our analysis showed that over 70% of the transformants tested remained mitotically stable even after five successive rounds of subculturing. We were able to detect the expression of EGFP and GUS reporter genes in the Ganoderma lucidum transformants by fluorescence imaging and histochemical staining assays respectively. Our results demonstrate a new transgenic approach that will facilitate Ganoderma lucidum research." @default.
- W3145660107 created "2021-04-13" @default.
- W3145660107 creator A5042870474 @default.
- W3145660107 creator A5060205920 @default.
- W3145660107 date "2012-01-01" @default.
- W3145660107 modified "2023-09-24" @default.
- W3145660107 title "Development of a simple and efficient transformation system for the basidiomycetous medicinal fungus Ganoderma lucidum" @default.
- W3145660107 cites W1910919787 @default.
- W3145660107 cites W1925751502 @default.
- W3145660107 cites W1968422424 @default.
- W3145660107 cites W1980195730 @default.
- W3145660107 cites W1981707478 @default.
- W3145660107 cites W1988868088 @default.
- W3145660107 cites W1992331398 @default.
- W3145660107 cites W2010803092 @default.
- W3145660107 cites W2015517788 @default.
- W3145660107 cites W2020559643 @default.
- W3145660107 cites W2027741551 @default.
- W3145660107 cites W2028275573 @default.
- W3145660107 cites W2028519017 @default.
- W3145660107 cites W2029633127 @default.
- W3145660107 cites W2035089399 @default.
- W3145660107 cites W2036245156 @default.
- W3145660107 cites W2042551683 @default.
- W3145660107 cites W2066221673 @default.
- W3145660107 cites W2078812903 @default.
- W3145660107 cites W2080015433 @default.
- W3145660107 cites W2093008319 @default.
- W3145660107 cites W2096879929 @default.
- W3145660107 cites W2107365509 @default.
- W3145660107 cites W2119782349 @default.
- W3145660107 cites W2164261080 @default.
- W3145660107 hasPublicationYear "2012" @default.
- W3145660107 type Work @default.
- W3145660107 sameAs 3145660107 @default.
- W3145660107 citedByCount "0" @default.
- W3145660107 crossrefType "journal-article" @default.
- W3145660107 hasAuthorship W3145660107A5042870474 @default.
- W3145660107 hasAuthorship W3145660107A5060205920 @default.
- W3145660107 hasConcept C104317684 @default.
- W3145660107 hasConcept C133479454 @default.
- W3145660107 hasConcept C173749725 @default.
- W3145660107 hasConcept C178050479 @default.
- W3145660107 hasConcept C204241405 @default.
- W3145660107 hasConcept C22744801 @default.
- W3145660107 hasConcept C2776162087 @default.
- W3145660107 hasConcept C2776618520 @default.
- W3145660107 hasConcept C2779656422 @default.
- W3145660107 hasConcept C2779678110 @default.
- W3145660107 hasConcept C2780874738 @default.
- W3145660107 hasConcept C2992468417 @default.
- W3145660107 hasConcept C31903555 @default.
- W3145660107 hasConcept C55493867 @default.
- W3145660107 hasConcept C59822182 @default.
- W3145660107 hasConcept C86803240 @default.
- W3145660107 hasConcept C89423630 @default.
- W3145660107 hasConceptScore W3145660107C104317684 @default.
- W3145660107 hasConceptScore W3145660107C133479454 @default.
- W3145660107 hasConceptScore W3145660107C173749725 @default.
- W3145660107 hasConceptScore W3145660107C178050479 @default.
- W3145660107 hasConceptScore W3145660107C204241405 @default.
- W3145660107 hasConceptScore W3145660107C22744801 @default.
- W3145660107 hasConceptScore W3145660107C2776162087 @default.
- W3145660107 hasConceptScore W3145660107C2776618520 @default.
- W3145660107 hasConceptScore W3145660107C2779656422 @default.
- W3145660107 hasConceptScore W3145660107C2779678110 @default.
- W3145660107 hasConceptScore W3145660107C2780874738 @default.
- W3145660107 hasConceptScore W3145660107C2992468417 @default.
- W3145660107 hasConceptScore W3145660107C31903555 @default.
- W3145660107 hasConceptScore W3145660107C55493867 @default.
- W3145660107 hasConceptScore W3145660107C59822182 @default.
- W3145660107 hasConceptScore W3145660107C86803240 @default.
- W3145660107 hasConceptScore W3145660107C89423630 @default.
- W3145660107 hasLocation W31456601071 @default.
- W3145660107 hasOpenAccess W3145660107 @default.
- W3145660107 hasPrimaryLocation W31456601071 @default.
- W3145660107 hasRelatedWork W11378451 @default.
- W3145660107 hasRelatedWork W1951588372 @default.
- W3145660107 hasRelatedWork W1991937901 @default.
- W3145660107 hasRelatedWork W2014376797 @default.
- W3145660107 hasRelatedWork W2024109080 @default.
- W3145660107 hasRelatedWork W2035631080 @default.
- W3145660107 hasRelatedWork W2036245156 @default.
- W3145660107 hasRelatedWork W2069575416 @default.
- W3145660107 hasRelatedWork W2074532830 @default.
- W3145660107 hasRelatedWork W2092280503 @default.
- W3145660107 hasRelatedWork W2321292940 @default.
- W3145660107 hasRelatedWork W2360161321 @default.
- W3145660107 hasRelatedWork W2381586685 @default.
- W3145660107 hasRelatedWork W2597696205 @default.
- W3145660107 hasRelatedWork W2770128063 @default.
- W3145660107 hasRelatedWork W2945264284 @default.
- W3145660107 hasRelatedWork W2951286683 @default.
- W3145660107 hasRelatedWork W2966506551 @default.
- W3145660107 hasRelatedWork W3021454331 @default.
- W3145660107 hasRelatedWork W3203398918 @default.
- W3145660107 isParatext "false" @default.
- W3145660107 isRetracted "false" @default.
- W3145660107 magId "3145660107" @default.
- W3145660107 workType "article" @default.