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- W3145735446 abstract "背景改变接枝通常比期望长拿的前面的十字形的系带(ACL ) 。基因治疗把一条激进的不同途径提供给接枝改变。在这研究，内部核糖体入口地点(怒火) 顺序被用来构造允许转变生长 factor-1 (TGF1 ) 和脉管的 endothelial 的合作表示的新 recombinant 侵入人体气管粘膜的病菌生长因素 165 (VEGF165 ) 基因(命名 Ad-VEGF165-1RES-TGF1 ) 。我们由包含 TGF1 的 ACL fibroblasts.Methods Adenoviral 向量在迁居和矩阵合成上调查了新侵入人体气管粘膜的病菌的效果， VEGF165 基因被构造。ACL 成纤维细胞从新西兰白兔子被获得。在 ACL 成纤维细胞暴露于 Ad-VEGF165-1RES-TGF1 以后， VEGF165 和 TGF1 蛋白质的表示被连接酶的 immunosorbent 试金(ELISA ) 和西方的弄污分析估计。VEGF165 和 TGF1 蛋白质的生物鉴定被西方的弄污的分析估计。ACL 成纤维细胞的增长和移植被估计由在 vitro 创伤闭合试金。骨胶原类型的基因表示在矩阵标记之中我，骨胶原类型，和 fibronectin mRNA 被结果显示出的即时 PCR.Results 估计包含 TGFI 和 VEGF165 基因的 recombinant 合作表示侵入人体气管粘膜的病菌向量的成功的构造。TGF1 和 VEGF165 的合作表示能在 TGF1 和 VEGF165 基因的矩阵 markers.Conclusion 合作表示之中导致 ACL 成纤维细胞和骨胶原类型，骨胶原类型，和 fibronectin mRNA 的高基因表示的相对快速、连续的增长由 ACL 成纤维细胞在迁居和矩阵合成上有更强大、有效的效果。" @default.
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- W3145735446 date "2008-01-01" @default.
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- W3145735446 title "Construction of recombinant adenovirus co-expression vector carrying the human transforming growth factor-β1 and vascular endothelial growth factor genes and its effect on anterior cruciate ligament fibroblasts" @default.
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