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- W3145855480 abstract "Objective To explore the feature of the ACh-sensitive potassium current in guinea pig cochlear outer hair cells.Methods Cochlear outer hair cells of guinea pigs(n=38)were isolated by collagenase type IV.Under the whole-cell patch mode,the ions nature and the pharmacological properties of the ACh-sensitive potassium current were investigated by applying the inhibitors of calcium-dependent potassium currents and the inhibitors of nicotinic ACh receptor.Results Following application of ACh, cochlear outer hair cells displayed a rapidly activating outward potassium current with a fast desensitized kinetic and a reversal(±s)potential of(-67.3±8.2)mV(n=10).At the holding potential of-50 mV,the current amplitude of ACh-sensitive potassium current activated by 100 μmol/L ACh was(506.6± 186.3)pA(n=9).ACh-sensitive potassium current was sensitive to TEA(tetraethylammonium chloride, 10 mmol/L)and potently inhibited by the small conductance calcium-dependent potassium current(SK) blocker,apamin(1μmol/L).Iberiotoxin(IBTX),the well-known blocker of big conductance calcium- dependent potassium current(BK),failed to inhibit the amplitude of the ACh-sensitive potassium current at the dose of 200 nmol/L.The dose for half-maximal response(EC_50)of the ACh-sensitive potassium current was(33.5±5.7)μmol/L(n=7).The ACh-sensitive potassium current was sensitive to the GABA (gamma-aminobutyric acid)-A receptor blocker,bicuculline, and strongly inhibited by the selective blocker of the α 9-nicotinic ACh receptor,strychnine.Strychnine and bicuculline showed the dose-dependent blocking effect with a half inhibition-maximal response(IC_50)of(22.3±2.6)nmol/L(n=7)and(1.2± 0.4)μmol/L(n=6),respectively.Conclusions This work provides direct evidences that the ACh- sensitive SK current was present on guinea pig cochlear outer hair cells.The activation of the ACh-sensitive SK current was most possibly mediated by a α 9-nicotinic ACh receptor." @default.
- W3145855480 created "2021-04-13" @default.
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- W3145855480 date "2007-01-01" @default.
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- W3145855480 title "Feature of ACh-sensitive potassium current in outer hair cells of guinea pig cochlea" @default.
- W3145855480 hasPublicationYear "2007" @default.
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