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- W3146036034 abstract "在接受者子孙识别相异的染色体并且在多倍性分析染色体部件，对棉花合适的一种 genomic 原位杂交(GISH ) 技术用增加的严厉条件被开发。增加的严厉条件是处于下列优化状态的四个因素的联合：堵住脱氧核糖核酸探查的 100:1 比率， 60% 形式酰胺洗答案， 43 pC 温度洗并且 13 min 洗。在从 Gossypium sturtianum (CC ) 把 gDNA 用作一根探针的这些特定的条件下面，强壮的杂交信号仅仅在混合 F 的体细胞从 C 染色体在染色体上被观察(G。hirsutum x G。sturtianum )(AtDtC ) 。因此， GISH 能在混血儿区别父母染色体。进一步，我们开发了多色 GISH 同时区别上述混血儿的三个染色体。结果反复显示了三个染色体，在， Dt，和 C，和各个与一种唯一的颜色染色体设定，使他们安逸识别。多色 GISH 的力量被六倍体混血儿 F 的分析证明(G。hirsutum x G。australe )(AtAtDtDtGG ) 。我们相信强大的多色 GISH 技术能广泛地被使用在多倍性分析染色体部件并且在接受者子孙识别相异的染色体。" @default.
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- W3146036034 date "2008-01-01" @default.
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- W3146036034 title "Establishment of a Multi-color Genomic in situ Hybridization Technique to Simultaneously Discriminate the Three Interspecific Hybrid Genomes in Gossypium" @default.
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