FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3146052662> ?p ?o ?g. }

• W3146052662 abstract "Objective To investigate if infection of human herpesvirus type 8 (HHV8) exists in Chinese patients with multiple myeloma (MM), and to identify the relationship between clinical manifestation and the viral load in bone marrow biopsy samples. Methods Real time quantitative PCR was conducted to define the viral load of HHV8 in bone marrow biopsy samples from 23 patients with MM. Nested PCR was conducted to amplify the KS330 233 Bam fragment of HHV8 from peripheral blood mononuclear cells, bone marrow aspirates and bone marrow biopsy samples. RT PCR was used to study the expression of suspected oncogenes: vIL 6 and vIRF 1 genes. Bone marrow biopsy samples of 5 patients with blood diseases other than MM and 5 patients with non blood system diseases were used as controls. PCR product of BCBL 1 cell line was used as positive control. Results (1) The positive rate of HHV8 in bone marrow biopsy samples from patients with MM was 69 6%(16/23)by real time fluorescence quantitative PCR, and 82 6%(19/23)by nested PCR. 16 bone marrow samples positive by fluorescence quantitative were positive too by nested PCR. However, 3 bone marrow samples positive by nested PCR were negative by fluorescence quantative. HHV8 was positive in only one of the 25 bone marrow aspirates from patients with MM (4%) and in none of the 20 peripharal blood samples from patients with MM. PCR showed negative result in 7 bone marrow aspirates taken form 13 MM patients with positive result by fluorescence quantative PCR. HHV8 was negative in bone marrow biopsy samples from patients with other diseases. HHV8 was negative in the peripheral blood samples from 16 MM patients, among which 9 out of the 11 cases with bone marrow biopsy samples taken showed HHV8 positive by fluorescence quatative PCR. Bone marrow biopsy samples of all patients with other blood diseases and non blood system diseases were HHV8 negative. RT PCR showed that among 12 MM patients the expression rate of vIRF 1 was 83.3%(10/12), and expression rate of vIL 6 was 0. The PCR positive products tallied with the sequence of HHV8 DNA. Clinical analysis showed that all of the patients with negative results had had chemotherapy. Conclusion There is a certain association between multiple myeloma pathogenesis and HHV8. In detection of HHVb, nested PCR is more sensitive than fluorescence quantitaative PCR. The viral gene vILR 1 plays a certain role in the pathogenesis of MM." @default.
• W3146052662 created "2021-04-13" @default.
• W3146052662 creator A5053886001 @default.
• W3146052662 date "2001-01-01" @default.
• W3146052662 modified "2023-09-28" @default.
• W3146052662 title "Quantitative analysis of human herpes virus type 8 and expression of its genes in patients with multiple myeloma" @default.
• W3146052662 hasPublicationYear "2001" @default.
• W3146052662 type Work @default.
• W3146052662 sameAs 3146052662 @default.
• W3146052662 citedByCount "0" @default.
• W3146052662 crossrefType "journal-article" @default.
• W3146052662 hasAuthorship W3146052662A5053886001 @default.
• W3146052662 hasConcept C104317684 @default.
• W3146052662 hasConcept C137061746 @default.
• W3146052662 hasConcept C142724271 @default.
• W3146052662 hasConcept C202751555 @default.
• W3146052662 hasConcept C203014093 @default.
• W3146052662 hasConcept C2775934546 @default.
• W3146052662 hasConcept C2776364478 @default.
• W3146052662 hasConcept C2780007613 @default.
• W3146052662 hasConcept C48023723 @default.
• W3146052662 hasConcept C55493867 @default.
• W3146052662 hasConcept C71924100 @default.
• W3146052662 hasConcept C86803240 @default.
• W3146052662 hasConceptScore W3146052662C104317684 @default.
• W3146052662 hasConceptScore W3146052662C137061746 @default.
• W3146052662 hasConceptScore W3146052662C142724271 @default.
• W3146052662 hasConceptScore W3146052662C202751555 @default.
• W3146052662 hasConceptScore W3146052662C203014093 @default.
• W3146052662 hasConceptScore W3146052662C2775934546 @default.
• W3146052662 hasConceptScore W3146052662C2776364478 @default.
• W3146052662 hasConceptScore W3146052662C2780007613 @default.
• W3146052662 hasConceptScore W3146052662C48023723 @default.
• W3146052662 hasConceptScore W3146052662C55493867 @default.
• W3146052662 hasConceptScore W3146052662C71924100 @default.
• W3146052662 hasConceptScore W3146052662C86803240 @default.
• W3146052662 hasLocation W31460526621 @default.
• W3146052662 hasOpenAccess W3146052662 @default.
• W3146052662 hasPrimaryLocation W31460526621 @default.
• W3146052662 hasRelatedWork W1608660340 @default.
• W3146052662 hasRelatedWork W1983832327 @default.
• W3146052662 hasRelatedWork W1991013814 @default.
• W3146052662 hasRelatedWork W2022676276 @default.
• W3146052662 hasRelatedWork W2077055182 @default.
• W3146052662 hasRelatedWork W2097896008 @default.
• W3146052662 hasRelatedWork W2118993092 @default.
• W3146052662 hasRelatedWork W2163658302 @default.
• W3146052662 hasRelatedWork W2366641518 @default.
• W3146052662 hasRelatedWork W2368399825 @default.
• W3146052662 hasRelatedWork W2370003717 @default.
• W3146052662 hasRelatedWork W2374336489 @default.
• W3146052662 hasRelatedWork W2376222477 @default.
• W3146052662 hasRelatedWork W2380572459 @default.
• W3146052662 hasRelatedWork W2396339269 @default.
• W3146052662 hasRelatedWork W2401246055 @default.
• W3146052662 hasRelatedWork W2409561074 @default.
• W3146052662 hasRelatedWork W2461793430 @default.
• W3146052662 hasRelatedWork W2883672538 @default.
• W3146052662 hasRelatedWork W2792352571 @default.
• W3146052662 isParatext "false" @default.
• W3146052662 isRetracted "false" @default.
• W3146052662 magId "3146052662" @default.
• W3146052662 workType "article" @default.