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- W3146066744 abstract "Equine infectious anemia virus(EIAV) is the etiological agent of equine infectious anemia(EIA). Donkey leukocyte attenuated equine infectious anemia virus(DLA EIAV)vaccine was the only successful lentivirus vaccine prepared in China ,which has been applied extensively in China against MA for twenty years. The vaccine was developed by passaging EIAV Liaoning strain(EIAV-L) in donkeys and obtained donkey-adapted equine infectious anemia virus(D-A EIAV) ,then attenuated it in donkey leukocytes. In this study, peripheral blood lymphocytes(PBL) were collected from a horse infected with EIAVL. The PBL DNAs were extracted. The EIAV-L proviral DNA was amplified in four parts, covering the whole proviral DNA sequence by polymerase chain reaction(PCR). Each of the four parts was cloned into the plasmid pBluescript SK, and obtained the four recombinant plasmid p2. 8, p2. 4, p3. 1 and p1. 2,re-spectively. Once the recombinants were comfirmed by restriction digestion. They were sequenced. The complete nucleotide sequence of EIAV-L provirus was determined by analyzing each of the four parts and connecting them as a whole. The genome of EIAV-L was 8235 bp in length,and G+C content was 38%. The analyzing results by the computer software DNASIS showed that the sequence of EIAV-L was 98. 4 % and 96.9% in homology with that of D-A EJAV and DLA EIAV, respectively. The high homology between these strains showed they were relationship. The homology between ELTV-L and D-A EIAV is higher than that between EIAV-L and DLA EIAV,this is consistent with the derivation process of DLA EIAV. Identical long terminal repeat(LTR)sequences which is 316bp in lengh were at both ends of EIAV-L provirus. The LTRs include U3, R, and U5 regions in turn,and the sizes of them were 197bp, 8Obp and 39bp, respectively. The genome of EIAV-L provirus had three long open reading frames(ORF) encoding gag,pol, and env genes, respectively. Gag gene, which was l200bp, located at 613-1912. Pal gene with 3402bp was at position 1708-5109. There was a termination codon in the env gene dividing it into two parts:envl of 699bp(nucleotides 5305-6003)and env2 of 1827bp(nucleotides 6073-7899). The provirus had three small ORFs,ie. Si ,52 and S3,with sizes of 153bp(nucleotides 5113-5265) ,204bp(nucleotides 5279-5482)and 402bp(nucleotides 7245-7646) ,respectively." @default.
- W3146066744 created "2021-04-13" @default.
- W3146066744 creator A5022679493 @default.
- W3146066744 date "2001-01-01" @default.
- W3146066744 modified "2023-09-23" @default.
- W3146066744 title "Complete Sequence of Proviral DNA of Equine Infectious Anemia Virus L Strain" @default.
- W3146066744 hasPublicationYear "2001" @default.
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