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- W3146074262 abstract "Objective To establish a method for detection of Clavibacter michiganensis subsp.Michiganensis by loop-mediated isothermal amplification protocol (LAMP).Methods Detection of 16S rRNA of Clavibacter michiganensis subsp.Michiganensis by a loop-mediated isothermal amplification protocol (LAMP) was evaluated in laboratory assays.LAMP amplified target DNA rapidly (1 h),isothermally (62 ℃) and with high-specificity based on four primers designed to recognize six independent sequences of target DNA.Results The LAMP primers did not react with suspensions of Pseudomonas corrugata,Pseudomonas solanacearum,and Pseudomonas syringae pv.tomato.The specificity of this LAMP assay was higher than PCR.Conclusion This method has convenient testing procedures,the required equipment is simple,and the results can be judged by the naked eye.It is sensitive than conventional PCR reaction and the results can be obtained earlier,so it has a greater advantage of the technology." @default.
- W3146074262 created "2021-04-13" @default.
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- W3146074262 date "2013-01-01" @default.
- W3146074262 modified "2023-09-25" @default.
- W3146074262 title "Detection of Clavibacter michiganensis subsp.Michiganensis by loop-mediated isothermal amplification" @default.
- W3146074262 hasPublicationYear "2013" @default.
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