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- W3146077945 abstract "Bcl-2 完了在许多人的肿瘤表示了并且涉及 tumorigenesis 和 chemoresistance.In 这研究，我们调查了发卡 Bcl-2 的禁止的效果小介入( si )在 cisplatin ( DDP )的 Bcl-2 基因的表示上的 RNA 抵抗的人的肺腺癌房间线 A549/DDP ，并且在 A549/DDP cells.Bcl-2 siRNA 和否定 siRNA 原生质标志的药促进感受性上的 Bcl-2 siRNA 的效果被构造并且稳定地 transfected 进 A549/DDP 房间。反向的抄写聚合酶链反应，免疫荧光显微镜学和蛋白质印迹分析被用来检测目标基因表达。自发的房间 apoptosis 被染色的氮蒽橘子和 ethidium 溴化物检测。到 DDP 和 diallyl 二硫化物(爸爸)的房间的药敏感被3-分析[ 4,5-dimethylthiazol-2-yl ]在 siRNA 马厩 transfectants 的 Bcl-2 mRNA 和蛋白质的 -2,5-diphenyltetrazolium 溴化物( MTT )试金和流动 cytometry.Expression 层次清楚地与否定 siRNA transfectants 相比被减少，未经治疗的 cells.MTT 结果显示 Bcl-2 transfectants 在有 0.2-200 亩 g/ml DDP 或 50-200 亩 M DADS.Flow cytometry 的处理以后的更高的房间抑制率在 Bcl-2 siRNA 房间揭示了增加的 apoptosis 。在 20 fig/ml DDP 或 100 个亩 M 爸爸的增加以后， siRNA 指向 Bcl-2 基因在到 DDP 和爸爸的 A549/DDP 房间，增加的自发的 apoptosis，和敏化的房间的明确地下面调整的基因表达。" @default.
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- W3146077945 date "2007-01-01" @default.
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- W3146077945 title "Bcl-2 Small Interfering RNA Sensitizes Cisplatin-resistant Human Lung Adenocarcinoma A549/DDP Cell to Cisplatin and Diallyl Disulfide" @default.
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