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- W3146082198 abstract "The insulin-like growth factors I and II(IGF-I and IGF-II) are important proteins involved in fish growth and development. Here, we report the isolation of IGF-II and expression analysis of IGFs in turbot Scophthalmus maximus, aiming to clarify their function in embryonic and larval development of fish. The deduced IGF-II gene is 808 bp in full length, which encodes a protein of 219 amino acids and is 93% similar with that of Paralichthys olicaceus in amino acid sequence. The tissue abundance and the expression pattern of IGFs in a turbot at early development stages were investigated via reverse transcription-polymer chain reaction. Result showed that the IGF-I and IGF-II genes were widely expressed in tissues of S. maximus. IGF-I was detected in all tissues except intestines with the highest level in liver, while IGF-II transcript presented in all tissues except muscle. At the stages of embryonic and larval development, the m RNA levels of IGFs sharply increased from the stage of unfertilized egg to post larva, followed by a decrease with larval development. However, there was an increase in IGF-I at the embryonic stage and IGF-II at the gastrula stage, respectively. These results suggested that IGFs play important roles in cell growth and division of the turbot. Our study provides reference data for further investigation of growth regulation in turbot, which can guarantee better understanding of the physiological role that IGFs play in fish." @default.
- W3146082198 created "2021-04-13" @default.
- W3146082198 creator A5080478625 @default.
- W3146082198 date "2015-01-01" @default.
- W3146082198 modified "2023-09-27" @default.
- W3146082198 title "Expression Analysis of the Insulin-Like Growth Factors I and II During Embryonic and Early Larval Development of Turbot(Scophthalmus maximus)" @default.
- W3146082198 hasPublicationYear "2015" @default.
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