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- W3146097387 abstract "objective To further characterize the HBb17 and M1 viruses isolated from Hainan Province by molecular biology.Methods The fragment containing partial E1 and 3' untranslated region of each of the viral isolate was amplified by RT-PCR, then subcloned.Recombinant was screened and sequenced.Analysis and comparison were carried out.Results 1.6kb fragment was amplified from HBb17 and a 1.3kb was from M1.Comparison of sequences showed,in 3' untranslated region the nucleotide homology between HBb17 and T48, the prototype of Ross River virus,is 99%,between M1 and Sagiyama virus is 98%. In the partial E1 sequence,the nucleotide(amino acid) homology between HBb17 and T48 is 99%(99%),between M1 and Sagiyama virus is 97%(99%),and between M1 and Getah virus is 94%(98%).Conclusion Sequence analysis showed HBb17 virus belongs to Ross River virus,M1 virus belongs to Sagiyama virus or Getah virus." @default.
- W3146097387 created "2021-04-13" @default.
- W3146097387 creator A5037499657 @default.
- W3146097387 date "2000-01-01" @default.
- W3146097387 modified "2023-09-24" @default.
- W3146097387 title "Cloning and primary analysis of 3'end genome of two alphaviruses isolated from Hainan Province of China" @default.
- W3146097387 hasPublicationYear "2000" @default.
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