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- W3146319049 abstract "瞄准:为了在有教养的 bovineaortic endothelial 的 apoptosis 上调查阿司匹林的效果,房间(BAEC ) 和信号小径在这包含了 process.Methods:在“ s 修改了鹰”的 Dulbecco 的 BAEC werecultured 和 passaged apoptotic 房间的 s 媒介文化 medium.Morphologic 变化 andquantification 在与 Hoechst 33258 .Cell 生存能力染色 thecells 以后用荧光显微镜被决定被测量by3-[ 4,5-dimefhylfhiazol-2-yl ]由 agarose 胶化 electrophoresis.Phospho-p38 的 -2,5-diphenyltetrazolium 溴化物( MTT ) method.DNA 破碎 wasvisualized 激活mitogen 的蛋白质 kinase ( MAPK )表示被西方的 blotting.Results 检测:在从 1X10 ~的低集中的阿司匹林( -10)mol/L 到 1x10 ~(-8) mol/L 减少了 apoptosis 和 p38 MAPK phosphorylation 由 H2O2 inBAEC 导致了,当高度阿司匹林开时( 1x10 ~(-7)-1x10~(-4) mol/L )导致了典型 apoptotic 变化 inBAEC 并且在 concentration-dependentmanner.SB203580 刺激了 phospho-p38 MAPK 的表示,一个特定的 p38 MAPK 禁止者,堵住了如此的 effects.Conclusion :阿司匹林在 BAEC 在 apoptosis 上展出 abiphasic 效果,减少在低集中的 apoptosis 和在高 concentration.p38 MAPK 的 inducingapoptosis 可以是调停的一个重要信号分子 effectsof 阿司匹林。" @default.
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- W3146319049 date "2007-01-01" @default.
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- W3146319049 title "Biphasic effect of aspirin on apoptosis of bovine vascular endothelial cells and its molecular mechanism" @default.
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