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- W3146377041 abstract "Rice seedlings were treated in Fe-deficient hydroponic solution for 1d,3d and 5 d respectively. Then soluble proteins in rice leaves were extracted with phenolic or TCA / acetone method, and protein dynamic changes were studied on the Fe-deficient stress by 2-DE analysis. The results depicted as follows:. First, using different pH IPG gel strip leaded to different separation effectiveness. Stained with coomassie brilliant blue, nearly 450 protein spots in a 2-DE gel were detected with the pH 3～10 gradient IPG strip, among which about 89% proteins were acidic ones. However, about 600 spots were tested in a 2-DE gel with the pH4～7 gradient IPG strip. In the 2-DE gel with pH4～7 IPG strip, 29 proteins were up-regulated, only 1 protein was down-regulated, 5 proteins were specially displayed. Second, the quality and quantity of the soluble proteins were different with different extraction methods. TCA technique was easy to operate and effective to distribute basic proteins on the basic apex of 2-DE gels. But the proteins extracted by TCA were difficult to resolve thoroughly. The proteins extracted by phenolic method were more quantitative and purer than that by TCA technique." @default.
- W3146377041 created "2021-04-13" @default.
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- W3146377041 date "2004-01-01" @default.
- W3146377041 modified "2023-09-25" @default.
- W3146377041 title "2-DE Method and proteomic map analysis in rice leaves under iron deficient stress" @default.
- W3146377041 hasPublicationYear "2004" @default.
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