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- W3146482381 abstract "The ribosomal internal transcribed spacer regions (ITS-1 and ITS-2) and mitochondrial gene fragments (16S rDNA and COI) of Crassostrea gigas were amplified via PCR, and the PCR products were ligated into T-vectors, cloned and sequenced. The nucleotide sequences of 543, 791, 530 and 700 bp from ITS-1, ITS-2, partial 16S rRNA gene and partial COI gene were obtained, respectively. The contents of A, T, G and C were 23.57%, 20.07%, 29.47% and 26.89% in ITS-1, 27.43%, 19.22%, 27.05% and 26.30% in ITS-2, 29.25%, 29.25%, 23.02% and 18.49% and 16S rDNA, and 22.71%, 39.43%, 20.43% and 17.43% in COI in turn. The primers of ITS-1 and ITS-2 proved to be very universal in a variety of mollusk species. The potential uses of these four sequences for species identification and relevant research of several closely related oyster species were discussed." @default.
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- W3146482381 date "2002-01-01" @default.
- W3146482381 modified "2023-09-23" @default.
- W3146482381 title "Sequencing of ribosomal internal transcribed spacer regions and mitochondrial gene fragments in Crassostrea gigas" @default.
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