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- W3146519005 abstract "Objective To explore the role of protein kinas e C (PKC) and activator protein-1 (AP-1) signal transduction cascade in the tran scription of interleukin-5 (IL-5 ) gene by peripheral blood T lymphocytes from asthmatic rat. Methods T lymphocytes were isolated and p urified from peripheral blood of each asthmatic Wistar male rat, and randomly di vided into 4 groups for culture. Group A, blank control; group B, PKC agonist ph orbol 12-myristate 13-acetate (PMA); group C, PMA and AP-1 cis-element decoy oligodeoxynucleotides (decoy ODNs); group D, PMA and AP-1 mutant decoy ODNs. T he decoy ODNs were transfected into the T cells of group C and D by cation lipos ome respectively. Reverse transcription-polymerase chain reaction (RT-PCR) was employed to assess IL-5 mRNA expression, and electrophoretic mobility shift as says (EMSA) for the activation of AP-1. Results The acti vation of AP-1 (86 777.22±2257.79) and the expression of IL-5 mRNA (0.8100±0 .0316) in T lymphocytes stimulated with PMA were significantly higher than these in blank control (20 708.54±968.04 and 0.3050±0.0129 respectively,P0.01 ), while the indexes (23 475.90±757.99 and 0.3450±0.0129 respectively) of T ly mphocytes stimulated with PMA and AP-1 decoy ODNs were significantly inhibited, as compared with group B (P0.01). The indexes (86 955.71±1600.38 and 0.81 75±0.0222 respectively) in T lymphocytes stimulated with PMA and AP-1 mutant d ecoy ODNs did not exhibit significant changes, by contrast with group B (P0 .05). The significant positive correlation was found between the activation of A P-1 and the expression of IL-5 mRNA (P0.01). Conclusion AP-1 may participate in the signal transduction of PKC-triggered transcr iption of IL-5 gene in asthmatic T lymphocytes. This suggests the activation of PKC/AP-1 signal transduction cascade of T lymphocytes may play an important ro le in the pathogenesis of asthma." @default.
- W3146519005 created "2021-04-13" @default.
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- W3146519005 date "2005-01-01" @default.
- W3146519005 modified "2023-09-23" @default.
- W3146519005 title "The role of protein kinase C and activator protein-1 signal cascade in the regu lation of interleukin-5 gene transcription in asthmatic rat" @default.
- W3146519005 hasPublicationYear "2005" @default.
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