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- W3146571821 abstract "CBF3 (CRT/DRE-binding 因素) 的完整的长度 cDNA 顺序被反向的抄写聚合酶链反应(RT-PCR ) 用基于在 GenBank 可得到的 CBF 基因设计的教材从 Vitis amurensis 克隆。顺序分析证明基因长有 854 bp，它的编码顺序包含了 720 bp，编码有 239 氨基酸和 AP2 的蛋白质结构的领域。CBF3 的分子的团被预言是 25.9 kDa，它的理论等电位的点是 7.02，脂肪族的索引是 59.29。蛋白质的平均 hydropathicity 是 ? 0.551。CBF3 的第三级的结构也被分析。包含 CBF3 基因的原核生物的表示向量 pGEX-4T-CBF3 被构造， CBF3 熔化蛋白质(52 kDa ) 与 1 mmol L 在正式就职以后在 Escherichia coli 被生产 ? 1 IPTG。进一步的研究被需要为改进工厂抵抗到象干旱和咸度那样的寒冷和另外的压力条件评估它的潜在的应用程序。" @default.
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- W3146571821 date "2010-01-01" @default.
- W3146571821 modified "2023-09-23" @default.
- W3146571821 title "Vitis amuerensis CBF3 Gene Isolation, Sequence Analysis and Expression" @default.
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