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- W3146653670 abstract "Summary: The synthesis of CCK-4 (H-Trp-Met-Asp-Phe-NH2) by using enzymes exclusively wasdescribed. As protection group for the amino group we used the Phenylacetyl group (Phac) whichhad been cleaved at the end of the synthesis with Penicillin G Amidase (PGA) without affectingthe peptide bonds. Thus, beginning with Phac-Trp-OH we had successfully synthesized the targetpeptide with following 4 enzymes, α-Chymotrypsin, Papain, Thermolysin and PGA in four reac-tion steps. All reactions were carried out in aqueous buffer in reasonable yields (＞65 %). FAB-MS or FD-MS verified the correct molecular mass of all peptides." @default.
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- W3146653670 date "2002-01-01" @default.
- W3146653670 modified "2023-09-24" @default.
- W3146653670 title "Synthesis of Cholecystokinin Peptide CCK—4 Exclusively by Enzymatic Methods" @default.
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