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- W3146664673 abstract "Objective To prepare monoclonal antibodies against human GL50 and to identify their biological functions. Methods Splenocytes were isolated from the mice immunized with Daudi on which GL50 is highly expressed, and then hybridized with murine plasmocytoma cells Sp2/0. The hybridoma specifically secreating antibody against human GL50 molecule were obtained by immunofluorescence staining and cytometry analysis through human GL50 transfectants. The ascetics induced were purified with protein G affinity chromatography. Isotope was verified by fast isotope strip. The specific recognition of McAb with the membrane protein GL50 was determined by indirect immunofluorescence staining and Western blot analysis. The expression pattern of GL50 molecule on different cells and cell lines was explored by flow cytometry. Cell counting, Trypan blue staining and MTT assay were employed to study the biological effects of McAbs on the proliferation of Daudi in vitro. Results Two hybridoma cell lines (12B11, 11C4) specifically secreted IgG2a against GL50. GL50 was predominately expressed on B lymphocyte cell lines. McAb 11C4 inhibited the proliferation of Daudi cells and reversed the stimulation effect of GL50-L cells on the proliferation of preactivated T cells. Conclusion McAbs 11C4 and 12B11 were specific anti-GL50 McAbs with high affinity to its receptor. McAb 11C4 manifested certain role in inducing the proliferation inhibitory effect of Daudi cells and preactivated T cells in vitro. They would be of significant value in basic research and clinical application." @default.
- W3146664673 created "2021-04-13" @default.
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- W3146664673 date "2004-01-01" @default.
- W3146664673 modified "2023-09-26" @default.
- W3146664673 title "Anti-GL50 McAbs and their biological functions" @default.
- W3146664673 hasPublicationYear "2004" @default.
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