Matches in SemOpenAlex for { <https://semopenalex.org/work/W3146778960> ?p ?o ?g. }
Showing items 1 to 99 of
99
with 100 items per page.
- W3146778960 endingPage "210" @default.
- W3146778960 startingPage "205" @default.
- W3146778960 abstract "构造 recombinant 的目的联系 adeno 的病毒共同表示人的脉管的上皮的生长因素 165 (hVEGF165 ) 并且骨头形态基因的蛋白质 7 (hBMP7 ) ，测量病毒 titer 并且验证再结合。AAV 没有助手的系统作为基础被过去常产生 recombinant AAV 的方法。plasmid pIRES 的怒火顺序被砍倒并且 subcloned 进包含向量 pAAV-MCS 构造 recombinant plasmid pAAV-MCSa-IRES-MCSb 的 ITR/ MCS。hVEGF165 和 hBMP7 基因被 PCR 放大并且分别地插入了到在上游的 MCSa 和下游的 MCSb。然后， recombinant plasmid pAAV-hVEGF165-IRES-hBMP7， pAAV-RC 和 pHelper 是进 AAV-293 房间的 co-transfected 完成包装的 rAAV-hVEGF165-IRES-hBMP7。标记 rAAV-IRES-GFP 的 GFP 被使用平行 plasmid pAAV-IRES-hrGFP 同时包装。包装的 AAV 的效率被监视在下面荧光灯显微镜和 recombinant 病毒的粒子从感染的 AAV-293 房间被收获。病毒 titer 被感染 AAV-HT1080 房间测量，并且 recombinant AAV-hVEGF165-IRES-hBMP7 被外长的兴趣基因的 PCR 验证。结果 Recombinant pAAV-hVEGF165-IRES-hBMP7 被两倍消化验证。在 AAV-293 的 GFP 表示能被观察在下面荧光灯在 transfection 和系统以后的显微镜 72 h 提供了 95% 的高收拾行李比率。recombinant 联系 adeno 的病毒有 5.5 脳的高 titer 1011 vp/ml，和 AAV-HT 1080 在 90% 的比率被感染。recombinant 病毒被外长的 hBMP7 和 hVEGF165 基因的 PCR 证实。结论 Recombinant rAAV-hVEGF165-IRES-hBMP7 成功地与高病毒 titer 被构造，它可以在 vitro 并且在 hVEGF165 和 hBMP7 合作表示的 vivo 实验提供基础为并且为骨头新生的基因治疗提供一个新方法。" @default.
- W3146778960 created "2021-04-13" @default.
- W3146778960 creator A5001314375 @default.
- W3146778960 creator A5008108990 @default.
- W3146778960 creator A5016915068 @default.
- W3146778960 creator A5018449935 @default.
- W3146778960 creator A5032245741 @default.
- W3146778960 creator A5047085432 @default.
- W3146778960 creator A5052925986 @default.
- W3146778960 creator A5063351633 @default.
- W3146778960 creator A5064289650 @default.
- W3146778960 creator A5067541916 @default.
- W3146778960 creator A5071540194 @default.
- W3146778960 creator A5082226881 @default.
- W3146778960 date "2008-01-01" @default.
- W3146778960 modified "2023-09-23" @default.
- W3146778960 title "Construction of recombinant adeno-associated virus vector co-expressing hVEGF165 and hBMP7 gene" @default.
- W3146778960 hasPublicationYear "2008" @default.
- W3146778960 type Work @default.
- W3146778960 sameAs 3146778960 @default.
- W3146778960 citedByCount "0" @default.
- W3146778960 crossrefType "journal-article" @default.
- W3146778960 hasAuthorship W3146778960A5001314375 @default.
- W3146778960 hasAuthorship W3146778960A5008108990 @default.
- W3146778960 hasAuthorship W3146778960A5016915068 @default.
- W3146778960 hasAuthorship W3146778960A5018449935 @default.
- W3146778960 hasAuthorship W3146778960A5032245741 @default.
- W3146778960 hasAuthorship W3146778960A5047085432 @default.
- W3146778960 hasAuthorship W3146778960A5052925986 @default.
- W3146778960 hasAuthorship W3146778960A5063351633 @default.
- W3146778960 hasAuthorship W3146778960A5064289650 @default.
- W3146778960 hasAuthorship W3146778960A5067541916 @default.
- W3146778960 hasAuthorship W3146778960A5071540194 @default.
- W3146778960 hasAuthorship W3146778960A5082226881 @default.
- W3146778960 hasConcept C104317684 @default.
- W3146778960 hasConcept C105580179 @default.
- W3146778960 hasConcept C149364088 @default.
- W3146778960 hasConcept C153911025 @default.
- W3146778960 hasConcept C159047783 @default.
- W3146778960 hasConcept C22744801 @default.
- W3146778960 hasConcept C2522874641 @default.
- W3146778960 hasConcept C2778107364 @default.
- W3146778960 hasConcept C32611913 @default.
- W3146778960 hasConcept C39060996 @default.
- W3146778960 hasConcept C40767141 @default.
- W3146778960 hasConcept C54009773 @default.
- W3146778960 hasConcept C54355233 @default.
- W3146778960 hasConcept C77430603 @default.
- W3146778960 hasConcept C81885089 @default.
- W3146778960 hasConcept C86803240 @default.
- W3146778960 hasConcept C92087593 @default.
- W3146778960 hasConceptScore W3146778960C104317684 @default.
- W3146778960 hasConceptScore W3146778960C105580179 @default.
- W3146778960 hasConceptScore W3146778960C149364088 @default.
- W3146778960 hasConceptScore W3146778960C153911025 @default.
- W3146778960 hasConceptScore W3146778960C159047783 @default.
- W3146778960 hasConceptScore W3146778960C22744801 @default.
- W3146778960 hasConceptScore W3146778960C2522874641 @default.
- W3146778960 hasConceptScore W3146778960C2778107364 @default.
- W3146778960 hasConceptScore W3146778960C32611913 @default.
- W3146778960 hasConceptScore W3146778960C39060996 @default.
- W3146778960 hasConceptScore W3146778960C40767141 @default.
- W3146778960 hasConceptScore W3146778960C54009773 @default.
- W3146778960 hasConceptScore W3146778960C54355233 @default.
- W3146778960 hasConceptScore W3146778960C77430603 @default.
- W3146778960 hasConceptScore W3146778960C81885089 @default.
- W3146778960 hasConceptScore W3146778960C86803240 @default.
- W3146778960 hasConceptScore W3146778960C92087593 @default.
- W3146778960 hasIssue "4" @default.
- W3146778960 hasLocation W31467789601 @default.
- W3146778960 hasOpenAccess W3146778960 @default.
- W3146778960 hasPrimaryLocation W31467789601 @default.
- W3146778960 hasRelatedWork W1983074066 @default.
- W3146778960 hasRelatedWork W1992270615 @default.
- W3146778960 hasRelatedWork W2348316764 @default.
- W3146778960 hasRelatedWork W2351233171 @default.
- W3146778960 hasRelatedWork W2355622788 @default.
- W3146778960 hasRelatedWork W2359409273 @default.
- W3146778960 hasRelatedWork W2368066453 @default.
- W3146778960 hasRelatedWork W2375771671 @default.
- W3146778960 hasRelatedWork W2387589889 @default.
- W3146778960 hasRelatedWork W2390819680 @default.
- W3146778960 hasRelatedWork W2391760144 @default.
- W3146778960 hasRelatedWork W2392882633 @default.
- W3146778960 hasRelatedWork W2393781575 @default.
- W3146778960 hasRelatedWork W2403035799 @default.
- W3146778960 hasRelatedWork W2409694381 @default.
- W3146778960 hasRelatedWork W2420122227 @default.
- W3146778960 hasRelatedWork W2468551101 @default.
- W3146778960 hasRelatedWork W251941095 @default.
- W3146778960 hasRelatedWork W2849110372 @default.
- W3146778960 hasRelatedWork W3147821787 @default.
- W3146778960 hasVolume "22" @default.
- W3146778960 isParatext "false" @default.
- W3146778960 isRetracted "false" @default.
- W3146778960 magId "3146778960" @default.
- W3146778960 workType "article" @default.