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• W3146819195 endingPage "12" @default.
• W3146819195 startingPage "1" @default.
• W3146819195 abstract "Little is known about the hormonal regulation of feline ovarian granulosa cell proliferation and steroidogenesis. The present study aimed to develop a hormone responsive granulosa cell culture system to measure steroidogenic and cell proliferation responses to help identify factors that might regulate ovarian function in queens. Five experiments were conducted each with 75 or more ovaries, three in spring and two in fall seasons. Granulosa cells were isolated and treated in vitro with various hormones in serum-free medium for 48 h after an initial 48 h plating in 10% fetal calf serum. In granulosa cells isolated from spring and fall collected feline ovaries, IGF1 alone and combined with FSH stimulated (P < 0.05) cell proliferation, whereas FSH alone had no effect (P > 0.10) on cell proliferation. Also, in granulosa cells collected in spring and fall, IGF1 alone and FSH alone increased (P < 0.05) estradiol production by severalfold, and a combination of FSH and IGF1 increased (P < 0.05) estradiol production above either FSH or IGF1 treatment alone. The FSH plus IGF1 treatment increased (P < 0.05) CYP19A1 mRNA abundance by 27-fold. In contrast, EGF decreased (P < 0.05) FSH plus IGF1-induced estradiol production by over 80% in granulosa cells of both spring and fall collected ovaries. In granulosa cells isolated from spring and fall collected ovaries, IGF1 plus FSH inhibited (P < 0.05) progesterone production. Melatonin increased (P < 0.05) FSH plus IGF1-induced cell proliferation and amplified (P < 0.05) the FSH plus IGF1-induced inhibition of progesterone production. However, melatonin and GH had no effect (P > 0.10) on estradiol production either alone or in combination with FSH plus IGF1 in both spring and fall. Prolactin, FGF9 and activin had no effect (P > 0.10) on cell proliferation or steroidogenesis. FGF2 decreased (P < 0.05) estradiol production without affecting progesterone production or cell numbers. Growth differentiation factor 9 (GDF9) increased (P < 0.05) progesterone production but had no effect (P > 0.10) on granulosa cell proliferation or estradiol production. In conclusion, the in vitro system described herewithin may be useful to assess and evaluate ovarian function in feline species and has identified EGF, FSH and IGF1 as major regulators of feline ovarian follicular function." @default.
• W3146819195 created "2021-04-13" @default.
• W3146819195 creator A5012254080 @default.
• W3146819195 creator A5028932355 @default.
• W3146819195 creator A5062150911 @default.
• W3146819195 creator A5070161872 @default.
• W3146819195 creator A5072575845 @default.
• W3146819195 date "2021-07-01" @default.
• W3146819195 modified "2023-10-15" @default.
• W3146819195 title "Effects of selected hormones and their combination on progesterone and estradiol production and proliferation of feline granulosa cells cultured in vitro" @default.
• W3146819195 cites W1162737367 @default.
• W3146819195 cites W1269873 @default.
• W3146819195 cites W1534117304 @default.
• W3146819195 cites W1554960389 @default.
• W3146819195 cites W1579836766 @default.
• W3146819195 cites W1966966464 @default.
• W3146819195 cites W1969685490 @default.
• W3146819195 cites W1971078400 @default.
• W3146819195 cites W1972428774 @default.
• W3146819195 cites W1979127741 @default.
• W3146819195 cites W1981122333 @default.
• W3146819195 cites W1982616308 @default.
• W3146819195 cites W1986654255 @default.
• W3146819195 cites W1987243507 @default.
• W3146819195 cites W1988564243 @default.
• W3146819195 cites W1992078851 @default.
• W3146819195 cites W1993198178 @default.
• W3146819195 cites W1997023957 @default.
• W3146819195 cites W1997096869 @default.
• W3146819195 cites W1997984830 @default.
• W3146819195 cites W2005318658 @default.
• W3146819195 cites W2006418970 @default.
• W3146819195 cites W2011750263 @default.
• W3146819195 cites W2013236842 @default.
• W3146819195 cites W2019908416 @default.
• W3146819195 cites W2021429556 @default.
• W3146819195 cites W2023696388 @default.
• W3146819195 cites W2026161667 @default.
• W3146819195 cites W2029288463 @default.
• W3146819195 cites W2029711968 @default.
• W3146819195 cites W2034008864 @default.
• W3146819195 cites W2035355757 @default.
• W3146819195 cites W2036483376 @default.
• W3146819195 cites W2037812119 @default.
• W3146819195 cites W2040824709 @default.
• W3146819195 cites W2041290762 @default.
• W3146819195 cites W2042523946 @default.
• W3146819195 cites W2043215244 @default.
• W3146819195 cites W2045437647 @default.
• W3146819195 cites W2048427763 @default.
• W3146819195 cites W2050874941 @default.
• W3146819195 cites W2054299742 @default.
• W3146819195 cites W2060572191 @default.
• W3146819195 cites W2061603582 @default.
• W3146819195 cites W2066929307 @default.
• W3146819195 cites W2068058863 @default.
• W3146819195 cites W2068364416 @default.
• W3146819195 cites W2069137928 @default.
• W3146819195 cites W2070798486 @default.
• W3146819195 cites W2073748870 @default.
• W3146819195 cites W2074585597 @default.
• W3146819195 cites W2074989666 @default.
• W3146819195 cites W2077024495 @default.
• W3146819195 cites W2079251901 @default.
• W3146819195 cites W2080146412 @default.
• W3146819195 cites W2083209415 @default.
• W3146819195 cites W2085337315 @default.
• W3146819195 cites W2087445097 @default.
• W3146819195 cites W2090660658 @default.
• W3146819195 cites W2091340945 @default.
• W3146819195 cites W2091929572 @default.
• W3146819195 cites W2092889645 @default.
• W3146819195 cites W2101246744 @default.
• W3146819195 cites W2105037784 @default.
• W3146819195 cites W2107096204 @default.
• W3146819195 cites W2107277218 @default.
• W3146819195 cites W2111269403 @default.
• W3146819195 cites W2113124658 @default.
• W3146819195 cites W2115360518 @default.
• W3146819195 cites W2123953793 @default.
• W3146819195 cites W2125768461 @default.
• W3146819195 cites W2127151362 @default.
• W3146819195 cites W2131391777 @default.
• W3146819195 cites W2134058674 @default.
• W3146819195 cites W2135089584 @default.
• W3146819195 cites W2136052981 @default.
• W3146819195 cites W2137647841 @default.
• W3146819195 cites W2138591344 @default.
• W3146819195 cites W2143932116 @default.
• W3146819195 cites W2149826618 @default.
• W3146819195 cites W2150227120 @default.
• W3146819195 cites W2150366724 @default.
• W3146819195 cites W2153272857 @default.
• W3146819195 cites W2153922745 @default.
• W3146819195 cites W2155990587 @default.
• W3146819195 cites W2159276134 @default.
• W3146819195 cites W2161276384 @default.
• W3146819195 cites W2161591640 @default.

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