Matches in SemOpenAlex for { <https://semopenalex.org/work/W3146875573> ?p ?o ?g. }
- W3146875573 endingPage "1673" @default.
- W3146875573 startingPage "1660" @default.
- W3146875573 abstract "SUMMARY Xyloglucan endotransglycosylase/hydrolase (XTH) enzymes play important roles in cell wall remodelling. Although previous studies have shown a pathway of evolution for XTH genes from bacterial licheninases, through plant endoglucanases (EG16), the order of development within the phylogenetic clades of true XTHs is yet to be elucidated. In addition, recent studies have revealed interesting and potentially useful patterns of transglycosylation beyond the standard xyloglucan–xyloglucan donor/acceptor substrate activities. To study evolutionary relationships and to search for enzymes with useful broad substrate specificities, genes from the ‘ancestral’ XTH clade of two monocots, Brachypodium distachyon and Triticum aestivum , and two eudicots, Arabidopsis thaliana and Populus tremula , were investigated. Specific activities of the heterologously produced enzymes showed remarkably broad substrate specificities. All the enzymes studied had high activity with the cellulose analogue HEC (hydroxyethyl cellulose) as well as with mixed‐link β‐glucan as donor substrates, when compared with the standard xyloglucan. Even more surprising was the wide range of acceptor substrates that these enzymes were able to catalyse reactions with, opening a broad range of possible roles for these enzymes, both within plants and in industrial, pharmaceutical and medical fields. Genome screening and expression analyses unexpectedly revealed that genes from this clade were found only in angiosperm genomes and were predominantly or solely expressed in reproductive tissues. We therefore posit that this phylogenetic group is significantly different and should be renamed as the group‐IV clade." @default.
- W3146875573 created "2021-04-13" @default.
- W3146875573 creator A5031536738 @default.
- W3146875573 creator A5060021214 @default.
- W3146875573 creator A5070002435 @default.
- W3146875573 date "2021-05-04" @default.
- W3146875573 modified "2023-10-15" @default.
- W3146875573 title "Enzymatic characterization of ancestral/group‐IV clade xyloglucan endotransglycosylase/hydrolase enzymes reveals broad substrate specificities" @default.
- W3146875573 cites W1537775205 @default.
- W3146875573 cites W1645370175 @default.
- W3146875573 cites W1889241553 @default.
- W3146875573 cites W1963554232 @default.
- W3146875573 cites W1968338503 @default.
- W3146875573 cites W1968357134 @default.
- W3146875573 cites W1968544926 @default.
- W3146875573 cites W1975814994 @default.
- W3146875573 cites W1979746994 @default.
- W3146875573 cites W1980843465 @default.
- W3146875573 cites W1983809516 @default.
- W3146875573 cites W1984420021 @default.
- W3146875573 cites W1993844043 @default.
- W3146875573 cites W1997785747 @default.
- W3146875573 cites W2001239076 @default.
- W3146875573 cites W2001396309 @default.
- W3146875573 cites W2002900187 @default.
- W3146875573 cites W2014194130 @default.
- W3146875573 cites W2015400144 @default.
- W3146875573 cites W2019847443 @default.
- W3146875573 cites W2021063639 @default.
- W3146875573 cites W2028251099 @default.
- W3146875573 cites W2031532105 @default.
- W3146875573 cites W2034481007 @default.
- W3146875573 cites W2043659757 @default.
- W3146875573 cites W2046542417 @default.
- W3146875573 cites W2049835121 @default.
- W3146875573 cites W2052090235 @default.
- W3146875573 cites W2053427298 @default.
- W3146875573 cites W2062455838 @default.
- W3146875573 cites W2066772628 @default.
- W3146875573 cites W2069102520 @default.
- W3146875573 cites W2078159980 @default.
- W3146875573 cites W2089480915 @default.
- W3146875573 cites W2094201044 @default.
- W3146875573 cites W2101746978 @default.
- W3146875573 cites W2102129193 @default.
- W3146875573 cites W2104057592 @default.
- W3146875573 cites W2104064305 @default.
- W3146875573 cites W2107158607 @default.
- W3146875573 cites W2107876387 @default.
- W3146875573 cites W2108992467 @default.
- W3146875573 cites W2109518880 @default.
- W3146875573 cites W2109579035 @default.
- W3146875573 cites W2115567294 @default.
- W3146875573 cites W2115783099 @default.
- W3146875573 cites W2118637251 @default.
- W3146875573 cites W2119404103 @default.
- W3146875573 cites W2120768613 @default.
- W3146875573 cites W2121368083 @default.
- W3146875573 cites W2121878319 @default.
- W3146875573 cites W2122809938 @default.
- W3146875573 cites W2123675258 @default.
- W3146875573 cites W2131868308 @default.
- W3146875573 cites W2135616804 @default.
- W3146875573 cites W2137015675 @default.
- W3146875573 cites W2141105436 @default.
- W3146875573 cites W2141292707 @default.
- W3146875573 cites W2142748378 @default.
- W3146875573 cites W2143581741 @default.
- W3146875573 cites W2146973550 @default.
- W3146875573 cites W2153496649 @default.
- W3146875573 cites W2153907785 @default.
- W3146875573 cites W2153912574 @default.
- W3146875573 cites W2157009395 @default.
- W3146875573 cites W2159080918 @default.
- W3146875573 cites W2161746138 @default.
- W3146875573 cites W2161869574 @default.
- W3146875573 cites W2161994403 @default.
- W3146875573 cites W2163410456 @default.
- W3146875573 cites W2306189402 @default.
- W3146875573 cites W2338901599 @default.
- W3146875573 cites W2496016021 @default.
- W3146875573 cites W2555982227 @default.
- W3146875573 cites W2608527701 @default.
- W3146875573 cites W2768477293 @default.
- W3146875573 cites W2770876949 @default.
- W3146875573 cites W2809610054 @default.
- W3146875573 cites W2886541918 @default.
- W3146875573 cites W2907238353 @default.
- W3146875573 cites W2921654417 @default.
- W3146875573 cites W2972315562 @default.
- W3146875573 cites W4244736178 @default.
- W3146875573 cites W4253164051 @default.
- W3146875573 doi "https://doi.org/10.1111/tpj.15262" @default.
- W3146875573 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33825243" @default.
- W3146875573 hasPublicationYear "2021" @default.
- W3146875573 type Work @default.
- W3146875573 sameAs 3146875573 @default.
- W3146875573 citedByCount "7" @default.