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- W3146953053 abstract "Aim:To define the role of enzymes involved in glutathione metabolism in 6- hydroxydopamine(6-OHDA)-induced glutathione alteration in primary cultured astrocytes.Methods:Total glutathione(GSx)levels were determined using the modified enzymatic microtiter plate assay.The mRNA levels of γ-glutamylcysteine synthetase(γGCS),γ-glutamyltransferase(γGT),glutathione peroxidase(GPx),GR (glutathione reductase),and glutathione transferases(GST)were determined us- ing RT-PCR.γGT activity was determined using 7GT assay kits.Results:In primary cultured astrocytes,6-OHDA induced a significant elevation of cellular GSx levels after treatment for 24 h.However,the GSx levels decreased after 24 h and the values were even lower than the value in the control group without 6- OHDA at 48 h.RT-PCR data showed that the mRNA levels of γGCS,the rate- limiting enzyme of γ-L-glutamyl-L-cysteinylglycine(GSH)synthesis,were increased by 6-OHDA after treatment for 24 h and 48 h;the mRNA levels of GPx,GR,and GST did not alter in 6-OHDA-treated astrocytes after treatment for 24 h and 48 h;and 6- OHDA increased the mRNA levels and the activity of TGT after treatment for 48 h, which induced a decrease in GSx levels,despite the up-regulation of γCS after exposure to 6-OHDA for 48 h.Conclusion:The change in γGCS correlated with the increase in GSH levels induced by 6-OHDA after treatment for 24 h.GSx levels decreased because of increased γGT mRNA levels and γGT activity induced by 6- OHDA after treatment for 48 h." @default.
- W3146953053 created "2021-04-13" @default.
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- W3146953053 date "2005-01-01" @default.
- W3146953053 modified "2023-09-24" @default.
- W3146953053 title "6-Hydroxydopamine-induced glutathione alteration occurs via glutathione enzyme system in primary cultured astrocytes" @default.
- W3146953053 hasPublicationYear "2005" @default.
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