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- W3146953216 abstract "Objective To explore the specific anti-leukemia immune response of CD8~+ cytotoxic T lymphocyte(CTL)derived from cord blood(CB)ex vivo and evaluate the feasibilities and values of the CTL for specific immunotherapy.Methods Dendritic cells(DC)were induced from mononuclear cells(MNC) by combination cytokines in 10 CB samples.Loading U937 cell lysate antigen on the mature DC,they could stimulate the lymphocytes of the same origin to generate CTL.MidiMACS was used to isolate CD8~+ CTL.A- nalysis of DC was performed by inverted microscopy,scanning electron microscopy and flow cytometry.Meth- yl thiazolyl tetrazolium(MTT)assay was used to evaluate the cytotoxicity of the CTL.Results Cocultured with GM-CSF,IL-4,TNF-α and PGE_2,CB-MNC could he induced into functional DC with typical morpholo- gy.The mean cytotoxicity of CD8~+ CTL to U937 cells was significant stronger than that of CD8~- CTL and TL at the same E:T ratios.The mean cytotoxicity rate of CD8~+ CTL to U937 cells was higher than that to K562 cells[(66.36±12.43)% vs(41.97±14.24)%]at E:T ratio of 40:1(P0.05).The cytotoxicity of CD8~- CTL to K562 cells showed no difference from that to U937 cells(P0.05).Conclusion Mature CB-DC loading U937 cell antigens could induce CB-T lymphocytes to gengerate leukemia-specific CD8~+ CTL. The cytotoxicity of the CD8~+ CTL is specific against U937 cells and is more potent than that of CD8~- CTL." @default.
- W3146953216 created "2021-04-13" @default.
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- W3146953216 date "2006-01-01" @default.
- W3146953216 modified "2023-09-24" @default.
- W3146953216 title "Specific induction of anti-leukemia effects by umbilical cord cell-derived CD8~+T cytotoxic lymphocytes" @default.
- W3146953216 hasPublicationYear "2006" @default.
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